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- 2025年07月06日
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- 库存：
1
- 供应商：
上海信裕
- 细胞形态：
上皮样
- 运输方式：
干冰运输
- 生长状态：
贴壁生长
人CXCL12/SDF-1蛋白 (isoform a，His标签)
产品信息
别称：
CXCL12，PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1
蛋白构建：
A DNA sequence encoding the human CXCL12 isoform alpha (P48061-2) (Lys 22-Lys 89) was expressed, with a polyhistidine tag at the N-terminus.
种属：
Human
表达宿主：
E. coli
人CXCL12/SDF-1蛋白 (isoform a，His标签)产品状态&运输说明：
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
质控检测
纯度：
> 90 % as determined by SDS-PAGE.
内毒素：
Please contact us for more information.
稳定性：
Samples are stable for up to twelve months from date of receipt at -70℃
预测N端：
Met
分子量：
The recombinant human CXCL12 consists of 84 amino acids and predicts a molecular mass of 10 KDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
人CXCL12/SDF-1蛋白 (isoform a，His标签)缓冲液：
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
使用指导
储存方法：
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶条件：
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.人CXCL12/SDF-1蛋白 (isoform a，His标签)
xy1711 XO 黄嘌呤氧化酶分析试剂盒 Xanthine Oxidase Assay 100 Tests in 96-well plat
xy1712 ALT "丙氨酸转氨酶分析试剂盒
" Alanine Transaminase Assay 100 Tests in 96-well plat
xy1713 GPDH 甘油醛3磷酸脱氢酶分析试剂盒 Glycerol-3-phosphate Dehydrogenase Assay 100 Tests in 96-well plat
xy1714 GST "谷胱甘肽S转移酶分析试剂盒
" Glutathione S-transferases Assay 100 Tests in 96-well plat
xy1715 ADH "乙醇脱氢酶分析试剂盒
" Alcohol Dehydrogenase Assay 100 Tests in 96-well plat
xy1716 ALDH " 醛脱氢酶分析试剂盒
" Aldehyde Dehydrogenase Assay 100 Tests in 96-well plat
xy1717
xy1718 阳离子阴离子检测试剂盒
xy1719 MGPA 孔雀绿磷酸盐检测试剂盒 Malachite Green Phosphate Assay 2500 tests/96 well plate
xy1720 CCA 比色法钙分析试剂盒 Colorimetric Calcium Assay 250 tests/96 well plate
xy1721 Ir 铁元素检测试剂盒 Iron Assay 100 tests
xy1722 代谢物检测试剂盒
xy1723 LAC L - 乳酸含量分析试剂盒 L-Lactate Assay 100 Tests in 96-well
xy1724 GLU 谷氨酸检测试剂盒 Glutamate Assay 100 Tests in 96-well plat
xy1725 NAD "NAD/NADH 含量检测试剂盒
" "NAD/NADH Assay
" 100 Tests in 96-well plate
xy1726 PYR 丙酮酸检测试剂盒 Pyruvate Assay 100 Tests
xy1727 G6P 6-磷酸葡萄糖检测试剂盒 Glucose-6-phosphate Assay 100 Tests
xy1728 GL 葡萄糖检测试剂盒 Glucose Assay 100 Tests
xy1729 UA 尿素检测试剂盒 Uric Acid Assay 100 Tests
xy1730 GLY 甘油检测试剂盒 Glycerol Assay 100 Tests
xy1731 TG "甘油三酸酯检测试剂盒
" Triglyceride Assay 100 Tests
xy1732 TGA "总谷胱甘肽检测试剂盒
" Total Glutathione Assay 100 Tests
xy1733 AA 氨检测试剂盒 Ammonia Assay 100 Tests
xy1734 GSH/GSSG 谷胱甘肽/氧化型谷胱甘肽比率检测试剂盒 GSH/GSSG Ratio Assay 100 Tests
xy1735 Cho 胆碱检测试剂盒 Choline Assay 100 Tests
xy1736 线粒体检测试剂盒
xy1737 MITOISO 线粒体分离试剂盒 Mitochondria Isolation Kit 100 reactions
xy1738 COX 细胞色素C氧化酶含量试剂盒 Cytochrome C Oxidase Assay 100Tests in cuvette
xy1739 CS 柠檬酸合成酶的检测试剂盒 Citrate Synthase Assay 100Tests in cuvette
xy1740 氧化与细胞应激试剂盒
xy1741 NO 一氧化氮色标法检测试剂盒 Colorimetric Nitric Oxide Assay 250 tests/96 well plate
xy1742 TACA 总抗氧化能力检测试剂盒 Total Antioxidant Capacity Assay 100 tests
xy1743 SOD 超氧化物歧化酶分析试剂盒 Superoxide Disutase Assay 100 tests
xy1744 CAT 过氧化氢酶活性分析试剂盒 Catalase Activity Assay 100 tests
xy1745 GPx 谷胱甘肽过氧化物酶分析试剂盒 Glutathione Peroxidase Assay 100 tests
xy1746 NAD "NAD/NADH 含量检测试剂盒
" "NAD/NADH Assay
" 100 Tests in 96-well plate
产品信息
别称：
CXCL12，PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1
蛋白构建：
A DNA sequence encoding the human CXCL12 isoform alpha (P48061-2) (Lys 22-Lys 89) was expressed, with a polyhistidine tag at the N-terminus.
种属：
Human
表达宿主：
E. coli
人CXCL12/SDF-1蛋白 (isoform a，His标签)产品状态&运输说明：
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
质控检测
纯度：
> 90 % as determined by SDS-PAGE.
内毒素：
Please contact us for more information.
稳定性：
Samples are stable for up to twelve months from date of receipt at -70℃
预测N端：
Met
分子量：
The recombinant human CXCL12 consists of 84 amino acids and predicts a molecular mass of 10 KDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
人CXCL12/SDF-1蛋白 (isoform a，His标签)缓冲液：
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
使用指导
储存方法：
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶条件：
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.人CXCL12/SDF-1蛋白 (isoform a，His标签)
xy1711 XO 黄嘌呤氧化酶分析试剂盒 Xanthine Oxidase Assay 100 Tests in 96-well plat
xy1712 ALT "丙氨酸转氨酶分析试剂盒
" Alanine Transaminase Assay 100 Tests in 96-well plat
xy1713 GPDH 甘油醛3磷酸脱氢酶分析试剂盒 Glycerol-3-phosphate Dehydrogenase Assay 100 Tests in 96-well plat
xy1714 GST "谷胱甘肽S转移酶分析试剂盒
" Glutathione S-transferases Assay 100 Tests in 96-well plat
xy1715 ADH "乙醇脱氢酶分析试剂盒
" Alcohol Dehydrogenase Assay 100 Tests in 96-well plat
xy1716 ALDH " 醛脱氢酶分析试剂盒
" Aldehyde Dehydrogenase Assay 100 Tests in 96-well plat
xy1717
xy1718 阳离子阴离子检测试剂盒
xy1719 MGPA 孔雀绿磷酸盐检测试剂盒 Malachite Green Phosphate Assay 2500 tests/96 well plate
xy1720 CCA 比色法钙分析试剂盒 Colorimetric Calcium Assay 250 tests/96 well plate
xy1721 Ir 铁元素检测试剂盒 Iron Assay 100 tests
xy1722 代谢物检测试剂盒
xy1723 LAC L - 乳酸含量分析试剂盒 L-Lactate Assay 100 Tests in 96-well
xy1724 GLU 谷氨酸检测试剂盒 Glutamate Assay 100 Tests in 96-well plat
xy1725 NAD "NAD/NADH 含量检测试剂盒
" "NAD/NADH Assay
" 100 Tests in 96-well plate
xy1726 PYR 丙酮酸检测试剂盒 Pyruvate Assay 100 Tests
xy1727 G6P 6-磷酸葡萄糖检测试剂盒 Glucose-6-phosphate Assay 100 Tests
xy1728 GL 葡萄糖检测试剂盒 Glucose Assay 100 Tests
xy1729 UA 尿素检测试剂盒 Uric Acid Assay 100 Tests
xy1730 GLY 甘油检测试剂盒 Glycerol Assay 100 Tests
xy1731 TG "甘油三酸酯检测试剂盒
" Triglyceride Assay 100 Tests
xy1732 TGA "总谷胱甘肽检测试剂盒
" Total Glutathione Assay 100 Tests
xy1733 AA 氨检测试剂盒 Ammonia Assay 100 Tests
xy1734 GSH/GSSG 谷胱甘肽/氧化型谷胱甘肽比率检测试剂盒 GSH/GSSG Ratio Assay 100 Tests
xy1735 Cho 胆碱检测试剂盒 Choline Assay 100 Tests
xy1736 线粒体检测试剂盒
xy1737 MITOISO 线粒体分离试剂盒 Mitochondria Isolation Kit 100 reactions
xy1738 COX 细胞色素C氧化酶含量试剂盒 Cytochrome C Oxidase Assay 100Tests in cuvette
xy1739 CS 柠檬酸合成酶的检测试剂盒 Citrate Synthase Assay 100Tests in cuvette
xy1740 氧化与细胞应激试剂盒
xy1741 NO 一氧化氮色标法检测试剂盒 Colorimetric Nitric Oxide Assay 250 tests/96 well plate
xy1742 TACA 总抗氧化能力检测试剂盒 Total Antioxidant Capacity Assay 100 tests
xy1743 SOD 超氧化物歧化酶分析试剂盒 Superoxide Disutase Assay 100 tests
xy1744 CAT 过氧化氢酶活性分析试剂盒 Catalase Activity Assay 100 tests
xy1745 GPx 谷胱甘肽过氧化物酶分析试剂盒 Glutathione Peroxidase Assay 100 tests
xy1746 NAD "NAD/NADH 含量检测试剂盒
" "NAD/NADH Assay
" 100 Tests in 96-well plate
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文献和实验相关实验
3.表达序列标签（EST）的研究现状 4.http://www.bbioo.com/bio101/2007/20990.htm otolaryngology To zhujoker：非常感谢，人类的基因序列和其中一种异构体蛋白序列已经完成，至于有没有isoform和几种isoform都不知道；小鼠的DNA是知道的，但是在ncbi中显示小鼠的mRNA序列尚未完全标注，其他的物种DNA都没有测完，从DNA及ensembl预测的mRNA序列来看是比较保守
His 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面： 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螯合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲液的pH、盐
Isoform-Selective Assays for Sphingosine Kinase Activity
Sphingosine kinases (SK) 1 and 2 are unique lipid kinases that phosphorylate sphingosine to form �sphingosine-1-phosphate (S1P). S1P is a bioactive molecule eliciting multiple effects both extracellularly via cell surface S1P receptors
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人CXCL12/SDF-1蛋白 (isoform a，His标签)
询价
