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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 保质期：
1-2年
- 英文名：
IPTG(异丙基β-D-硫代半乳糖苷)
- 库存：
100⁺瓶
- 供应商：
上海睿安生物13611631389
- CAS号：
367-93-1
- 规格：
10g/瓶
Sigma货号I5502-10g现货IPTG异丙基β-D-1-硫代半乳糖吡喃糖苷13611631389上海睿安生物
形式powder
包装:1g、5g、10g in poly bottle.
应用:IPTG通常用于需要诱导β-半乳糖苷酶活性的克隆程序。它与X-Gal或Bluo-Gal一起用于重组细菌菌落的蓝白斑筛选，其诱导lac操纵子在大肠杆菌中的表达。IPTG通过与lacI阻遏物结合，并改变其构象起作用，其阻止β-半乳糖苷酶编码基因lacZ的阻遏作用。
其他说明:非代谢性半乳糖类似物。
重悬:将IPTG溶于水，然后经无菌过滤，配制成储存液(0.1M)。指示板中IPTG最终浓度应为0.2mM。
同行评审论文
The effects of chemical interactions and culture history on the colonization of structured habitats by competing bacterial populations.
Simon van Vliet et al.
BMC microbiology, 14, 116-116 (2014-06-03)
Bacterial habitats, such as soil and the gut, are structured at the micrometer scale. Important aspects of microbial life in such spatial ecosystems are migration and colonization. Here we explore the colonization of a structured ecosystem by two neutrally labeled
Multimeric recombinant M2e protein-based ELISA: a significant improvement in differentiating avian influenza infected chickens from vaccinated ones.
Farshid Hadifar et al.
PloS one, 9(10), e108420-e108420 (2014-10-21)
Killed avian influenza virus (AIV) vaccines have been used to control H5N1 infections in countries where the virus is endemic. Distinguishing vaccinated from naturally infected birds (DIVA) in such situations however, has become a major challenge. Recently, we introduced the
Mutations in DCPS and EDC3 in autosomal recessive intellectual disability indicate a crucial role for mRNA decapping in neurodevelopment.
Iltaf Ahmed et al.
Human molecular genetics, 24(11), 3172-3180 (2015-02-24)
There are two known mRNA degradation pathways, 3' to 5' and 5' to 3'. We identified likely pathogenic variants in two genes involved in these two pathways in individuals with intellectual disability. In a large family with multiple branches, we
Transcriptomic changes of Legionella pneumophila in water.
Laam Li et al.
BMC genomics, 16, 637-637 (2015-08-27)
Legionella pneumophila (Lp) is a water-borne opportunistic pathogen. In water, Lp can survive for an extended period of time until it encounters a permissive host. Therefore, identifying genes that are required for survival in water may help develop strategies to
Assessment of Mycobacterium_ tuberculosis pantothenate kinase vulnerability through target knockdown and mechanistically diverse inhibitors.
B K Kishore Reddy et al.
Antimicrobial agents and chemotherapy, 58(6), 3312-3326 (2014-04-02)
Pantothenate kinase (PanK) catalyzes the phosphorylation of pantothenate, the first committed and rate-limiting step toward coenzyme A (CoA) biosynthesis. In our earlier reports, we had established that the type I isoform encoded by the coaA gene is an essential pantothenate
Sigma授权代理●询订13611631389上海睿强生物&上海睿安生物
一、╳※试剂※╳PBMC分离服务:PBMC/人外周血单个核细胞/PBMC细胞/人外周血单个核细胞/冻存PBMC/hPBMC单个核细胞
二、╳※试剂※╳:
常规血清优势供应:VivaCell货号C04001-500血清、Access货号51HI血清、Gemini货号900-108血清、Gemini血清、Gibco血清(10099-141澳洲血清、10270-106南美血清、16000-044血清)、BI血清BI货号04-001-1ACS、Sigma货号F8318-500ml澳洲胎牛血清、Sigma货号F8687澳洲胎牛血清、Sigma货号F2442-500ml特级胎牛血清、ScienCell货号0500澳洲胎牛血清、Cegrogen货号A0500-3010胎牛血清、Cegrogen货号B0500-5010新生牛血清、康源血清
培养基(包括无血清)、冻存液(包括无血清)，包括:友康Yocon
Merck(Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)正式授权代理、博奥龙Biodragon独dú家总代理、EFL独dú家总代理、碧云天Beyotime代理、Proteintech、Active Motif正式授权代理
☆☆☆☆☆☆Beckman货号A63881纯化磁珠Agencourt AMPure XP(60ml/瓶)/A63882(450ml/瓶)；Beckman货号B23317核酸片段筛选试剂盒Agencourt SPRIselect(5ml/瓶)
分子类:Biotium货号41003的GelRed染料、货号31000的EvaGreen染料、Zymo Research、Qiagen
饲料类:Research Diets、Dyets
三、╳※耗材※╳:
洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材/KirGen耗材、博麦迪(常规的)各类试剂瓶
Bioplastics
Thermo Fisher Scientific的很多产品线，包括旗下的:
一、Nunc耗材
二、QSP耗材(各类吸头/滤芯吸头、离心管、PCR管等)
三、Nalgene耗材仪器
四、FisherBrand耗材仪器(含各类盖玻片载玻片等)
五、Lab_Serv耗材仪器
六、Finnpipette移液器(如：F1系列、F2系列、F3系列等等各类型号的移液器)
七、Abgene耗材
畅销款:我们是常备有大量现货的
可请随时咨询:万奎13611631389(可以请加微信)
※╳√√✓✔√✓✔√✓✔上海睿安生物科技有限公司成立于2009年，专注于为生命科学和生物技术领域的客户提供优质的产品与服务。代理了多家欧美知名品牌，如美国Thermo Fisher耗材(包括旗下Nunc、QSP、Nalgene、FisherBrand、LabServ耗材仪器、Finnpipette移液器、Abgene耗材，等等)、默克Merck-Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)、Zymo Research、洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材、博奥龙Biodragon独dú家总代理、EFL独dú家总代理、碧云天Beyotime代理、Eppendorf、友康Yocon、Research Diets高脂饲料，IBL、BioVendor、Mercodia、ShiBayaji代谢类产品、Axygen-Corning长期大量耗材备货、BioCoat畅销款长期备货，Access货号51HI血清、Gemini血清、Gibco血清，BI血清现货、ScienCell血清、Sigma血清、Moregate血清、Cegrogen血清、Pierce发光试剂、Fermentas、Invitrogen、Proteintech、Active Motif、Abcam、CST、BD、SAB、R&D systems、Beckman、Qiagen、BD Pharmingen、Biolegend、Jackson、Miltenyi、MP Biomedicals、Biotium更好的荧光试剂产品等品牌。睿安生物始终将“提供更好的产品和服务”作为企业发展的目标和努力的方向，并将通过优质的售前与售后服务，始终如一地为客户提供高质量的产品和服务，我们也希望能得到相关领域中更多老师和同仁们的指导和鞭策，为共创优秀品牌而努力奋斗！询订╳直线13611631389微信号
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- 询问日期
文献和实验该产品被引用文献
Sigma货号I5502-10g现货IPTG异丙基β-D-1-硫代半乳糖吡喃糖苷13611631389上海睿安生物
形式powder
包装:1g、5g、10g in poly bottle.
应用:IPTG通常用于需要诱导β-半乳糖苷酶活性的克隆程序。它与X-Gal或Bluo-Gal一起用于重组细菌菌落的蓝白斑筛选，其诱导lac操纵子在大肠杆菌中的表达。IPTG通过与lacI阻遏物结合，并改变其构象起作用，其阻止β-半乳糖苷酶编码基因lacZ的阻遏作用。
其他说明:非代谢性半乳糖类似物。
重悬:将IPTG溶于水，然后经无菌过滤，配制成储存液(0.1M)。指示板中IPTG最终浓度应为0.2mM。
同行评审论文
The effects of chemical interactions and culture history on the colonization of structured habitats by competing bacterial populations.
Simon van Vliet et al.
BMC microbiology, 14, 116-116 (2014-06-03)
Bacterial habitats, such as soil and the gut, are structured at the micrometer scale. Important aspects of microbial life in such spatial ecosystems are migration and colonization. Here we explore the colonization of a structured ecosystem by two neutrally labeled
Multimeric recombinant M2e protein-based ELISA: a significant improvement in differentiating avian influenza infected chickens from vaccinated ones.
Farshid Hadifar et al.
PloS one, 9(10), e108420-e108420 (2014-10-21)
Killed avian influenza virus (AIV) vaccines have been used to control H5N1 infections in countries where the virus is endemic. Distinguishing vaccinated from naturally infected birds (DIVA) in such situations however, has become a major challenge. Recently, we introduced the
Mutations in DCPS and EDC3 in autosomal recessive intellectual disability indicate a crucial role for mRNA decapping in neurodevelopment.
Iltaf Ahmed et al.
Human molecular genetics, 24(11), 3172-3180 (2015-02-24)
There are two known mRNA degradation pathways, 3' to 5' and 5' to 3'. We identified likely pathogenic variants in two genes involved in these two pathways in individuals with intellectual disability. In a large family with multiple branches, we
Transcriptomic changes of Legionella pneumophila in water.
Laam Li et al.
BMC genomics, 16, 637-637 (2015-08-27)
Legionella pneumophila (Lp) is a water-borne opportunistic pathogen. In water, Lp can survive for an extended period of time until it encounters a permissive host. Therefore, identifying genes that are required for survival in water may help develop strategies to
Assessment of Mycobacterium_ tuberculosis pantothenate kinase vulnerability through target knockdown and mechanistically diverse inhibitors.
B K Kishore Reddy et al.
Antimicrobial agents and chemotherapy, 58(6), 3312-3326 (2014-04-02)
Pantothenate kinase (PanK) catalyzes the phosphorylation of pantothenate, the first committed and rate-limiting step toward coenzyme A (CoA) biosynthesis. In our earlier reports, we had established that the type I isoform encoded by the coaA gene is an essential pantothenate
Sigma授权代理●询订13611631389上海睿强生物&上海睿安生物
一、╳※试剂※╳PBMC分离服务:PBMC/人外周血单个核细胞/PBMC细胞/人外周血单个核细胞/冻存PBMC/hPBMC单个核细胞
二、╳※试剂※╳:
常规血清优势供应:VivaCell货号C04001-500血清、Access货号51HI血清、Gemini货号900-108血清、Gemini血清、Gibco血清(10099-141澳洲血清、10270-106南美血清、16000-044血清)、BI血清BI货号04-001-1ACS、Sigma货号F8318-500ml澳洲胎牛血清、Sigma货号F8687澳洲胎牛血清、Sigma货号F2442-500ml特级胎牛血清、ScienCell货号0500澳洲胎牛血清、Cegrogen货号A0500-3010胎牛血清、Cegrogen货号B0500-5010新生牛血清、康源血清
培养基(包括无血清)、冻存液(包括无血清)，包括:友康Yocon
Merck(Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)正式授权代理、博奥龙Biodragon独dú家总代理、EFL独dú家总代理、碧云天Beyotime代理、Proteintech、Active Motif正式授权代理
☆☆☆☆☆☆Beckman货号A63881纯化磁珠Agencourt AMPure XP(60ml/瓶)/A63882(450ml/瓶)；Beckman货号B23317核酸片段筛选试剂盒Agencourt SPRIselect(5ml/瓶)
分子类:Biotium货号41003的GelRed染料、货号31000的EvaGreen染料、Zymo Research、Qiagen
饲料类:Research Diets、Dyets
三、╳※耗材※╳:
洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材/KirGen耗材、博麦迪(常规的)各类试剂瓶
Bioplastics
Thermo Fisher Scientific的很多产品线，包括旗下的:
一、Nunc耗材
二、QSP耗材(各类吸头/滤芯吸头、离心管、PCR管等)
三、Nalgene耗材仪器
四、FisherBrand耗材仪器(含各类盖玻片载玻片等)
五、Lab_Serv耗材仪器
六、Finnpipette移液器(如：F1系列、F2系列、F3系列等等各类型号的移液器)
七、Abgene耗材
畅销款:我们是常备有大量现货的
可请随时咨询:万奎13611631389(可以请加微信)
※╳√√✓✔√✓✔√✓✔上海睿安生物科技有限公司成立于2009年，专注于为生命科学和生物技术领域的客户提供优质的产品与服务。代理了多家欧美知名品牌，如美国Thermo Fisher耗材(包括旗下Nunc、QSP、Nalgene、FisherBrand、LabServ耗材仪器、Finnpipette移液器、Abgene耗材，等等)、默克Merck-Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)、Zymo Research、洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材、博奥龙Biodragon独dú家总代理、EFL独dú家总代理、碧云天Beyotime代理、Eppendorf、友康Yocon、Research Diets高脂饲料，IBL、BioVendor、Mercodia、ShiBayaji代谢类产品、Axygen-Corning长期大量耗材备货、BioCoat畅销款长期备货，Access货号51HI血清、Gemini血清、Gibco血清，BI血清现货、ScienCell血清、Sigma血清、Moregate血清、Cegrogen血清、Pierce发光试剂、Fermentas、Invitrogen、Proteintech、Active Motif、Abcam、CST、BD、SAB、R&D systems、Beckman、Qiagen、BD Pharmingen、Biolegend、Jackson、Miltenyi、MP Biomedicals、Biotium更好的荧光试剂产品等品牌。睿安生物始终将“提供更好的产品和服务”作为企业发展的目标和努力的方向，并将通过优质的售前与售后服务，始终如一地为客户提供高质量的产品和服务，我们也希望能得到相关领域中更多老师和同仁们的指导和鞭策，为共创优秀品牌而努力奋斗！询订╳直线13611631389微信号
相关实验
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
祝贺〖上海雅裕 〗成为 Signalway Antibody 品牌 (SAB) 的独家总代理。 促销【 ABCD 】细则如下： 【 A 】 买两支 SAB 抗体免费获得 8G 优盘 活动时间：即日起，至 2011 年
的底物进行筛选。我们通过离体印迹磷酸化作用来确认可能的底物。总的来说，我们的方法能筛选植物蛋白激酶用于进一步的分析，随后的体内实验对评估它们的生理作用是必不可少的 [ 13，24，25 ]。2. 材料 2.1 非变性条件下重组激酶的纯化 ( 1 ) 培养细胞的培养基：含 100 μg/ml 氨苄青霉素，15 μg/ml 卡那霉素，2% 葡萄糖的 2YB 或 LB 培养基。 ( 2 ) 异丙基 β-D-硫代半乳糖苷（IPTG ) 。 ( 3 ) 非变性裂解液：300 mmol
( 1 ) PRSETB 细菌表达质粒（Invitrogen ) : 用于 Ni-次氮基三乙酸（NTA ) 琼脂糖蛋白纯化的 His-tag；用于选择的氨苄青霉素和用于诱导蛋白质表达的异丙基-β-D-硫代半乳糖吡喃糖苷（IPTG )。 ( 2 ) pcDNA3 哺乳动物瞬时表达质粒（Invitrogen ) : 巨细胞病毒启动子和用于细菌选择的氨苄青霉素抗性基因。 2.2 生物化学和生物物理鉴定 ( 1 ) Luria-Bertani ( LB ) 培养基：10 g 胰蛋白胨，5 g 酵母
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Sigma货号I5502-10g现货IPTG异丙基β-D-1-硫代半乳糖吡喃糖苷13611631389上海睿安生物
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