
MP-007 Minute™ 哺乳动物细胞/组织线粒体分离试
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- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 英文名:
Minute™ Mitochondrial Isolation Kit for Mammalian Cells and Tissues
- 规格:
50 Preps/4 Preps
| 规格: | 50 Preps | 产品价格: | ¥6206.0 |
|---|---|---|---|
| 规格: | 4 Preps | 产品价格: | ¥630.0 |
MinuteTM 哺乳动物细胞/组织线粒体分离试剂盒
目录号:MP-007
描述
哺乳动物细胞/组织线粒体提取试剂盒由优化的缓冲液系统和2.0ml离心管柱组成。可以快速的从哺乳动物细胞/组织中提取线粒体蛋白。专利离心管柱技术使用方便简单,高产,与其他商业试剂盒相比,可以样品处理范围更广泛,可处理5-50million/样品,并且可以得到完整的线粒体。缓冲液系统不含表面活性剂和EDTA,无需匀浆和组织研磨,操作时间<30分钟。
原理:细胞/组织通过Buffer A致敏,然后细胞以Z字形路径通过离心管柱,在这个过程中细胞膜会破裂。进而通过差速离心和密度离心分离出完整的线粒体,整个过程无需匀浆和超高速离心。
应用
提取出的线粒体可以应用于SDS-PAGE,immunoblottings,ELISA,IP,膜蛋白结构分析,2-D,酶活性检测和其他应用

Figure 1. Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with MinuteTM Mitochondria Isolation Kit.
A. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3 5, total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver tissues respectively. Lanes 2, 4, 6, isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues respectively.
B. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-cytochrome C reductase core protein (ab96333, abcam, Cambridge, MA) and anti-lamin B1 (ab16048,a nuclear envelope marker protein. abcam, Cambridge, MA).
C. Densitometry measurement of mitochondrial marker signals.
产品特点
简单,快速,性价比高
得率高,可获得更完整的线粒体
处理样品范围更广
缓冲液系统不含表面活性剂和EDTA
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and show the characteristic membrane blebbing (bubble formation)seen in cells dying via apoptosis.Promega公司的DeadEnd™比色法细胞凋亡检测系统能标记片断化的DNA,而这种片段化的DNA被视为是细胞凋亡的典型生化特征之一。DeadEnd™比色法细胞凋亡检测系统是一种在组织切片与培养细胞中标记凋亡细胞核的理想方法,与此同时,还可以做形态学上的评估。本文中,将展示此种方法在细胞凋亡的原位细胞, 动物模型及多种病理学组织切片中的应用。抗Fas
SARS-CoV-2、甲型流感病毒(infuenza A virus) 或单纯疱疹病毒1 (HSV-1) 的唾液样本分别与 PBS、CANDOR的SafetyTector™ S按照 1:4 比例混合,室温孵育1 分钟 (n = 3)。孵育后,样本进行滴定并 被加入到Vero E6 (SARS-Cov-2 和 HSV-1) 、MDCK (infuenza A virus) 细胞中进行培养。4-7天后,采用Reed-Muench 法测 定病毒感染性。结果显示,对于所有测试的三种病毒,使用此方法
柱式法 Minute TM 胞质胞核分离试剂盒操作方法:A. 悬浮细胞样品(包括植物,细菌,酵母和真菌制备的原生质体)1.500Xg,3 分钟低速离心收集细胞,用预冷的 PBS 清洗一次2. 将细胞转移到 1.5 ml 离心管中,3000 rpm 离心 1-5 分钟,弃去上清。3. 按表格 1 加入适量的胞浆提取缓冲液(请注意样品及裂解液的比例,以达到最佳效果), 涡旋大力震荡 15 秒, 冰上孵育 5 分钟, 混匀. 接转胞质胞核分离步骤。B. 贴壁细胞1. 贴壁细胞生长至融合度 90











