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ER-036 Minute™ 动物细胞/组织内质网富集试剂盒

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  • ¥6282
  • Invent已认证
  • ER-036
  • 美国
  • 2026年01月06日
    48h发货
    24h回复
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    • 用户评价
    • 文献和实验
    • 技术资料
    • 英文名

      Minute™ ER Enrichment Kit For Tissues and Cultured Cells

    • 保存条件

      4℃

    • 规格

      20 Preps

    MinuteTM 动物细胞/组织内质网富集试剂盒

     

    目录号:ER-036

      描述   内质网是连接核膜和质膜功能的主要膜结构。内质网在真核细胞蛋白转运的胞外途径中起重要作用。在细胞质中合成的蛋白质被运送到内质网,囊泡将蛋白质运送到高尔基体,然后与质膜融合。传统分离内质网的方法是基于密度梯度超离心,需要大量的起始样品,方法繁琐,耗时,交叉污染严重。目前,所有内质网分离的商用试剂盒都是基于上世纪70年代开发的方法。与市场上的内质网分离试剂盒不同,我们的内质网分离试剂盒使用离心管柱技术,简单、快速,只需少量的起始培养细胞或组织,不需要使用杜恩斯匀浆管和超高速离心,就能从培养的细胞/组织中分离出天然ER(主要是粗面内质网)。整个操作可以在大约2h内完成。

    产品细节图片1
    储存温度:
    4℃

     
    试剂盒组分(20 Preps):
    1. 缓冲液 A 20ml
    2. 缓冲液 B 1ml
    3. 缓冲液 C 1ml
    4. 缓冲液 D 10ml
    5. 塑料研磨棒 2 根
    6. 离心管柱 20 个
    7. 收集管 20 个

     

    重要产品信息:
    1. 操作前请仔细阅读整个操作说明。将离心管柱插入接收管形成套管放置于冰上预冷。
    2. 离心机请调整成离心力 Rcf(g)模式,所有离心步骤都需要在 4℃室温下或者低温离心机中进行。
    3. 涉及蛋白磷酸化的研究,务必在使用前将磷酸酶抑制剂加入缓冲液 A 中。如果担心蛋白质降解,可以在使用前在缓冲液 A 中加入蛋白酶抑制剂(请按照蛋白酶或磷酸酶抑制剂母液比例添加,例如母液是 100x,添加时按照 1:100 添加,1ml 缓冲液 A 添加 10ul 抑制剂)。
    4. 推荐使用 BCA 方法测定蛋白浓度。
    5. 请勿使用液氮,匀浆仪等研磨方式,研磨方式请按说明书操作。分离的 ER 的产率和纯度可能会根据特定的细胞/组织类型和所用起始材料的量有所变化。可以通过优化以实现最佳结果
    (具体优化方案见下面的技术说明)。

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      图标文献和实验
      该产品被引用文献

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      4.Zhang, L., Li, R., Geng, R., Wang, L., Chen, X. X., Qiao, S., & Zhang, G. (2022). Tumor Susceptibility Gene 101 (TSG101) Contributes to Virion Formation of Porcine Reproductive and Respiratory Syndrome Virus via Interaction with the Nucleocapsid (N) Protein along with the Early Secretory Pathway. Journal of Virology,

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      8.Wang, Y., Cao, X., Liu, P., Zeng, W., Peng, R., Shi, Q., ... & Wang, H. (2022). KCTD7 mutations impair the trafficking of lysosomal enzymes through CLN5 accumulation to cause neuronal ceroid lipofuscinoses. Science Advances.

      9.Ruiz-Rodado, V., Lita, A., & Larion, M. (2022). Advances in measuring cancer cell metabolism with subcellular resolution. Nature Methods, 1-16.

      10.Ghosh, D. K., Udupa, P., Shrikondawar, A. N., Bhavani, G. S., Shah, H., Ranjan, A., & Girisha, K. M. (2022). Mutant MESD links cellular stress to type I collagen aggregation in osteogenesis imperfecta type XX. Matrix Biology.

      11.Chiu, C. C., Weng, Y. H., Yeh, T. H., Lu, J. C., Chen, W. S., Li, A. H. R., ... & Wang, H. L. (2023). Deficiency of RAB39B Activates ER Stress-Induced Pro-apoptotic Pathway and Causes Mitochondrial Dysfunction and Oxidative Stress in Dopaminergic Neurons by Impairing Autophagy and Upregulating α-Synuclein. Molecular Neurobiology, 1-23.

      12.Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.

      13.Liu, Y. Y., Bai, J. S., Liu, C. C., Zhou, J. F., Chen, J., Cheng, Y., & Zhou, B. (2023). The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly. Journal of Virology,

      14.Xiao, X., Shi, J., He, C., Bu, X., Sun, Y., Gao, M., ... & Zhang, J. (2023). ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy. Nature Communications.

      15.Li, Y., Li, K., Pan, T., Xie, Q., Cheng, Y., Wu, X., ... & Cui, S. (2023). Translocation of IGF-1R in endoplasmic reticulum enhances SERCA2 activity to trigger Ca2+ ER perturbation in hepatocellular carcinoma. Acta Pharmaceutica Sinica B.

      16.Zhu, Y., Lei, L., Wang, X., Jiang, Q., Loor, J. J., Kong, F., ... & Li, X. (2023). Low abundance of insulin-induced gene 1 contributes to SREBP-1c processing and hepatic steatosis in dairy cows with severe fatty liver. Journal of Dairy Science.

      17.Zhou, Q., Yang, Y., Xu, Z., Deng, K., Zhang, Z., Hao, J., ... & Li, Y. P. (2023). ATAD1 inhibits hepatitis C virus infection by removing the viral TA‐protein NS5B from mitochondria. EMBO reports, 

      18.Wang, W., Tan, J., Liu, X., Guo, W., Li, M., Liu, X., ... & Zhou, Q. (2023). Cytoplasmic Endonuclease G promotes nonalcoholic fatty liver disease via mTORC2-AKT-ACLY and endoplasmic reticulum stress. Nature Communications, 

      19.Guo, C. G., Sun, R., Wang, X., Yuan, Y., Xu, Y., Li, S., ... & Zhang, X. (2023). Intestinal SURF4 is essential for apolipoprotein transport and lipoprotein secretion. Molecular Metabolism,

      20.Xiong, F. R., Lu, J., Zhu, J. J., Zhao, R. X., Zhang, Y. C., & Yang, J. K. (2024). KCNH6 is essential for insulin secretion by regulating intracellular ER Ca2+ store. The FASEB Journal, 38(4),

      21.Bi, Y. Y., Chen, Q., Yang, M. Y., Xing, L., & Jiang, H. L. (2024). Nanoparticles targeting mutant p53 overcome chemoresistance and tumor recurrence in non-small cell lung cancer. Nature Communications, 

      22.Bansal, R., Torres, M., Hunt, M., Wang, N., Chatzopoulou, M., Manchanda, M., ... & Wikstrom, J. D. (2024). Role of the mitochondrial protein cyclophilin D in skin wound healing and collagen. JCI insight.

      23.Chiu, C. C., Chen, Y. L., Weng, Y. H., Liu, S. Y., Li, H. L., Yeh, T. H., & Wang, H. L. (2024). Downregulation of Protease Cathepsin D and Upregulation of Pathologic α-Synuclein Mediate Paucity of DNAJC6-Induced Degeneration of Dopaminergic Neurons. International Journal of Molecular Sciences, 

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      27.Aldonza MBD, Cha J, Yong I, Ku J, Sinitcyn P, Lee D, Cho RE, Delos Reyes RD, Kim D, Kim S, Kang M, Ku Y, Park G, Sung HJ, Ryu HS, Cho S, Kim TM, Kim P, Cho JY, Kim Y. Multi-targeted therapy resistance via drug-induced secretome fucosylation. Elife.

      28.Wu CY, Cheng CW, Kung CC, Liao KS, Jan JT, Ma C, Wong CH. Glycosite-deleted mRNA of SARS-CoV-2 spike protein as a broad-spectrum vaccine. Proc Natl Acad Sci U S A. 2022 Mar 1;119(9):e2119995119. doi: 10.1073/pnas.2119995119.

      29.Hongtao Shen, Yuying Xie, Yan Wang, Yusheng Xie, Yongxiang Wang,Zhenyan Su, Laixi Zhao, Shi Yao, Xiaoling Cao, Jinglan Liang, Junrui Long, Rimei Zhong, Jinfeng Tang, Sijie Wang, Liangqing Zhang, Xiaojing Wang, Björn Stork, Lili Cui & Wenxian Wu (2025): The ER protein CANX (calnexin)-mediated autophagy protects against alzheimer disease,Autophagy, DOI: 10.1080/15548627.2024.2447206

      30.Donghyun Kang; Jeeyeon Lee; Geunho Yook; Seok Hoo Jeong; Jungkwon Shin; et al.(2025).Regulation of senescence-associated secretory phenotypes in osteoarthritis by cytosolic UDP-GlcNAc retention and O-GlcNAcylation.Nature Communications.DOI 10.1038/s41467-024-55085-1

      31. Anqi Luo; Rui Wang; Jingwen Gong; Shuting Wang; Chuan Yun; et al.(2025).Syntaxin 17 Translocation Mediated Mitophagy Switching Drives Hyperglycemia‐Induced Vascular Injury. Advanced Science.DOI 10.1002/advs.202414960

      32.Tingting Li; Rihua He; Qing Tian; Tianhao Huang; Honghui Jiang; et al.(2025).Cancer-associated fibroblast-derived circKLHL24 drives perineural invasion in pancreatic cancer via dual regulation of the sec31a-CXCL12 axis.Journal of Experimental & Clinical Cancer Research.DOI 10.1186/s13046-025-03489-2

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      研选
      ER-036 Minute™ 动物细胞/组织内质网富集试剂盒
      ¥6282