SM-005-P Minute™ 植物膜蛋白提取试剂盒

Minute^TM 植物膜蛋白提取试剂盒

目录号：SM-005-P

  描述   植物膜蛋白占植物细胞总蛋白的很小一部分，但是在植物生理学中起着非常重要的作用。传统的植物膜蛋白分离纯化方法是蔗糖密度梯度离心法和双液相法。这些方法虽然比较有效，但是需要超高速离心和大量的起始原材料，操作过程十分繁琐和费时。本试剂盒解决了植物膜蛋白提取中的难点。植物组织首先通过缓冲液 A 中致敏，匀浆，然后通过离心管柱，在此过程中匀浆的组织通过柱子特有的 Z 字形通路后细胞膜被切割成大小相等的碎片，后续无需超高速离心，通过差速离心和密度离心可将天然的质膜蛋白从未破裂的细胞，细胞核，细胞质和细胞器的混合物中分离出来。在每次实验中仅需使用相同量的起始材料，离心力和离心时间，即可高度富集膜蛋白，并保证一致性良好。整个操作过程大约 1 小时可以完成。

  应用  

试剂盒用于快速从植物组织中分离天然膜蛋白，可应用于 SDS-PAGE，immunoblottings，ELISA，IP，膜蛋白质结构分析，2-D，酶活性测定及其他应用。

    Protocol

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参考文献  

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