CD44 (CD44) (AA 3-1) 抗体

CD44 (CD44) (AA 3-1) 抗体抗原
CD44 (CD44)
别名 AU023126, AW121933, AW146109, HERMES, Ly-24, Pgp-1, CD44A, METAA, RHAMM, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1
抗原表位
其他选择 AA 3-1
适用
其他选择 小鼠
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (5034-44-2)
标记
其他选择 非结合性
应用范围
其他选择 Cytotoxicity Test (CyTox), 流式细胞分析技术（FACS）
Pubmed 4个引用可参考
规格 0.5 mL
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb114216
产品细节 CD44 (CD44) (AA 3-1) 抗体
免疫原 B6 - Ly-1a spleen DONOR: BALB/c spleen FUSION PARTNER: P3-NS1-1-Ag4(NS1/1)
克隆位点 5034-44-2
亚型 IgG2a
特异性 This monoclonal antibody recognizes the murine alloantigen Ly 24.2. CD44 (Ly 24B.2, Pgp-1.2) is a 95 kDa glycoprotein previously known as phagocytic glycoprotein -1. It has a wide tissue distribution and is found on bone marrow derived cells, lymphocytes and non - lymphoid tissue such as brain, liver, and kidney. There is variation in Ly 24 expression between mice strains. Generally expression by Ly 24.1 strains is high while in Ly 24.2 it is lower (2). The Ly 24 antigen is expressed by T lymphocytes during primary antigen stimulation. This antigen can be used as a marker to identify activated or memory T cells (3,4).
纯化方法 Ascites
目标详细情况 CD44 (CD44) (AA 3-1) 抗体
别名 CD44
背景 CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1
基因ID 12505
NCBI登录号 NP_001034240
UniProt P15379
研究领域 Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle
使用细节 CD44 (CD44) (AA 3-1) 抗体
应用备注 Flow cytometry. (see Protocols)Method for Determining percent of positive cells in a poulation. (see Protocols)Method for Depleting a cells poulation of positive lymphocytes. (see Protocols)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare cell suspension in Media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte® M Cell Separation Medium. 2. Wash 2 times. 3. Resuspend cells to 1 x 10e6 cells in approximately 50 µl Media A in a microcentrifugetube (ie. 50 µl of cells resuspended to 2 x 10e7 cells / ml). (THE CONTENTS OF 1 TUBEREPRESENTS 1 TEST). 4. To each tube add 50 µl of 1/500 dilution of this Ab(final dilution 1/1000). 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody CLCC30201 (Goat anti-mouse IgG(H+L)-FITC conjugate)@ 1: 700. 9. Incubate tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in Media B. 11. Resuspend the cell pellet in 50 µl ice cold Media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg / ml in phosphate buffered saline. (This stains dead cells byintercalating DNA). MEDIA: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% bovine serum albumin + sodium azide (100 µlof 2M sodium azide in 100 mls). RESULTS - TISSUE DISTRIBUTION: PROCEDURE: as aboveANTIBODY CONCENTRATION: 1: 1000MOUSE STRAIN: C57BL/6CELL SOURCE: PERCENT STAININGThymus: 8. 8%Spleen: 36. 1%Lymph Node: 17. 9%
限制 仅限研究用
贮存及处理 CD44 (CD44) (AA 3-1) 抗体
状态 Liquid
溶解方式 Reconstitute with 0.5 mL of cold distilled water.
注意事项 Use of Sodium Azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.