CD44 (CD44) 抗体

CD44 (CD44) 抗体
抗原
CD44 (CD44)
别名 AU023126, AW121933, AW146109, HERMES, Ly-24, Pgp-1, CD44A, METAA, RHAMM, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1
适用
其他选择 小鼠
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (KM81)
标记
其他选择 FITC
CD44 (CD44) 抗体应用范围
其他选择 流式细胞分析技术（FACS）
Pubmed 4个引用可参考
规格 0.3 mg
发货至 中国 (更改)
产品细节
免疫原 Bone Marrow Derived Stromal cells (clone BMS2). Donor: Lou/MN Rat. Fusion Partner: SP2/0.
克隆位点 KM81
亚型 IgG2a
特异性 This monoclonal antibody recognizes a 95 kDa glycoprotein found on most hematopoietic cells (1). It is thought to be important in the regulation of migratory properties of lymphocytes during development and the regulation of the interaction with bone marrow stromal cells during hematopoiesis (2,3). CD44 functions as a receptor for hyaluronate, although some cells expressing CD44 do not bind hyaluronate (3,4). This antibody has been shown to inhibit the growth of lymphoid and myeloid cells on long term bone marrow cultures (3). It also blocks the adhesive interactions of B cell hybridomas to a cloned stromal line or to hyaluronate coated dishes (4).
纯化方法 Protein G Chromatography
目标详细情况
别名 CD44
CD44 (CD44) 抗体背景 CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1
基因ID 12505
NCBI登录号 NP_001034240
UniProt P15379
研究领域 Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle
使用细节
应用备注 Flow cytometry. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 1. 0 - 0. 5 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsIsotypic Control: FITC Rat IgG2aResults - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg /10e6 cellsStrains Tested: BALB/c, CBA/J, C3H/He, C57BL/6, SWRPositive: BALB/c, CBA/J, C3H/He, C57BL/6, SWRNegative: noneIMMUNOHISTOCHEMISTRY:
限制 仅限研究用
贮存及处理
浓度 0.1 mg/mL
缓冲液 PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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