Murine leukemia viruses (MuLVs or MLVs) are retroviruses known for their ability to infect and cause cancer in murine hosts and other vertebrates. Moloney murine leukemia virus-based retroviral vectors are widely used for gene transfer to various target cells. Recently discovered in humans, xenotropic murine leukemia virus-related virus (XMRV) is closely related to murine leukemia viruses (e.g. Gag p30 core antigens share 96% identity). Early studies have linked XMRV to prostate cancer and chronic fatigue sydrome; however, new findings have disputed these claims. Introduction
Cell Biolabs’ QuickTiter™ MuLV Core Antigen ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the MuLV core antigen protein (Gag p30). The kit has a detection sensitivity limit of 300 pg/mL MuLV p30. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and MuLV samples.
MuLV Core Antigen标准曲线:
重组MuLV-p30蛋白的纯化:Lane 1: MW STDs; Lane 2: E.Coli Whole Lysate (-IPTG); Lane 3: E.Coli Whole Lysate (+IPTG); Lane 4: Crude Lysate; Lane 5: Lysate after Ni-NTA beads; Lane 6: Bead Wash; Lane 7: Elution Fraction for Recombinant MuLV p30 Standard.