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- 2025年07月15日
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- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
欣博盛生物
- 检测范围：
/
- 检测方法：
竞争法ELISA
- 应用：
/
- 适应物种：
other
- 标记物：
/
- 样本：
血清，血浆等
- 规格：
1*96wells
Enzo中国授权代理商 产品名称：Corticosterone ELISA kit 货号：ADI-900-097 规格：1*96wells
Enzo Life Sciences, Inc.隶属于全球上市公司Enzo Biochem，Inc.，总部设于美国纽约的法明代尔。Enzo在研发、生产、销售生物研究试剂方面拥有30多年的丰富成功经验，可提供多种细胞活性荧光染料、蛋白、抗体、多肽、小分子、标签探针染料及试剂盒等，用于研究目标的鉴定、高通量分析、基因表达分析、核酸检测、蛋白组学研究及细胞检测等。
更多详情请联系Enzo中国授权代理商欣博盛生物
Alternative size available: ADI-901-097 (5x96 wells)
Sensitive measurement of Corticosterone, detecting as little as 27 pg/ml
High throughput format with results in 3 hours for up to 39 samples in duplicate
Broad dynamic range
Species independent
Routinely used to monitor stress levels in zoo animals
The Corticosterone EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. The Corticosterone EIA kit has low cross-reactivity with related steroids. The broad dynamic range makes this kit ideal for a wide variety of sample matrices from any species.
Product Details
Sensitivity: 27.0 pg/ml (range 32 - 20,000 pg/ml)
Assay Time: 3 hours
Applications: ELISA, Colorimetric detection
Application Notes: For the quantitative determination of Corticosterone in culture supernatants, feces, plasma, serum, and saliva from any species. Cited sample types include cell lysate, urine and whole blood.
Wavelength: 405 nm
Species reactivity: Species independent
Crossreactivity: Corticosterone (100%), Deoxycorticosterone (21.3%), Desoxycorticosterone (21.0%), Progesterone (0.46%), Testosterone (0.31%), Tetrahydrocorticosterone (0.28%), Aldosterone (0.18%), Cortisol (0.046%) and <0.03%: Pregnenolone, Estradiol, Cortisone, 11-dehydrocorticosterone acetate
Shipping: Blue Ice Not Frozen
Long Term Storage: +4°C
Contents: DxS IgG Microtiter plate, Assay buffer 15 concentrate, Steroid displacement reagent, Conjugate, Antibody, Wash buffer concentrate, Standard, pN-pp Substrate, Stop solution
Scientific Background: Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Corticosterone production has been shown to increase with stress, with elevated corticosterone levels being associated with impairment of long term memory retrieval and following burn injury. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns.
Technical Info/Product Notes: Cited samples:
Hormone ELISAs: Cited Samples
Wildlife Endocrinology: Cited Sample Types
Protocol: Suggested Protocol for Small Volumes of Serum/Plasma Samples
Let all solutions come to room temp before use.
Aliquot 10µl of each sample into microfuge tube.
Make 1ml 1:100 Steroid Displacement Reagent (SDR) solution (immediately before use) in deionized water or PBS (not assay buffer)
Add 10µl 1:100 SDR to each sample tube.
Vortex, let stand >5 minutes before diluting with EIA buffer.
Add 380µl EIA assay buffer to each plasma tube, vortex.
Final dilution is ~1:40 (This final dilution may not be appropriate for every sample; thus, optimal dilution must be determined by each end user for their experimental sample group.)
Regulatory Status: RUO - Research Use Only
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文献和实验Adolescent intermittent ethanol exposure enhances adult stress effects in male rats: K.L. Healey, et al.; Pharmacol. Biochem. Behav. 223, 173513 (2023),
Anti-depressive-like and cognitive impairment alleviation effects of Gastrodia elata Blume water extract is related to gut microbiome remodeling in Apo-/- mice exposed to unpredictable chronic mild stress: H.S. Huang, et al.; J. Ethnopharmacol. 302, 115872 (2023),
Bank Voles Show More Impulsivity in IntelliCage Learning Tasks than Wood Mice: M. Jörimann, et al.; Neuroscience 510, 157 (2023),
Cannabinoid Receptor 2 Blockade Prevents Anti-Depressive-like Effect of Can-nabidiol Acid Me-thyl Ester in Female WKY Rats: D.H. Shoval, et al.; Int. J. Mol. Sci. 24, 3828 (2023),
Chemically inducible split protein regulators for mammalian cells: E. Rihtar, et al.; Nat. Chem. Biol. 19, 64 (2023),
Chronic stress induced loudness hyperacusis, sound avoidance and auditory cortex hyperactivity: S. Manohar, et al.; Hear. Res. 431, 108726 (2023),
Circadian rhythm shifts and alcohol access in adolescence synergistically increase alcohol preference and intake in adulthood in male C57BL/6 mice: D. Gonzalez, et al.; Behav. Brain Res. 438, 114216 (2023),
Effects of dietary protein levels on performance, nitrogen excretion, and odor emission of growing pullets and laying hens: Y.J. Heo, et al.; Poult. Sci. 102, 102798 (2023),
Effects of Intermittent Fasting on Hypothalamus-Pituitary-Thyroid Axis, Palatable Food Intake, and Body Weight in Stressed Rats: C.G. Luna, et al.; Nutrients 15, 1164 (2023),
Environmental enrichment accentuates glucose-induced feeding suppression and glial cell line-derived neurotrophic factor gene expression in the hypothalamus of mice: M.S. Aldhshan, et al.; Nutr. Neurosci. (2023),
Environmental signals perceived by the brain abate pro-metastatic monocytes by dampening glucocorticoids receptor signaling: M.M. Canali, et al.; Cancer Cell Int. 23, 15 (2023),
Evaluation of an Extract Derived from the Seaweed Ascophyllum nodosum to Reduce the Negative Effects of Heat Stress on Broiler Growth and Stress Parameters: G.S. Archer, et al.; Animals 13, 259 (2023),
Fermented Perilla frutescens Ameliorates Depression-like Behavior in Sleep-Deprivation-Induced Stress Model: H.J. Lee, et al.; Int. J. Mol. Sci. 24, 622 (2023),
Gut microbiota mediated inflammation, neuroendocrine and neurotrophic functions involved in the antidepressant-like effects of diosgenin in chronic restraint stress: J.J. Cui, et al.; J. Affect. Disord. 321, 242 (2023),
Impact of host stress on the replication rate of Plasmodium take it easy to avoid malaria recurrences: R. Pigeault, et al.; Front. Ecol. Evol. (2023), Application(s): Canary (bird) plasma,
Implications of atrazine concentrations in drinking water from Ijebu-North, Southwest Nigeria on the hypothalamic-pituitary-adrenal axis: F. Owagboriaye, et al.; Drug Chem. Toxicol. (2023),
Inhibition of c-Jun in AgRP neurons increases stress-induced anxiety and colitis susceptibility: F. Jiao, et al.; Commun. Biol. 6, 50 (2023),
Integrating Reference Intervals into Chimpanzee Welfare Research: J.C. Whitham, et al.; Animals (Basel) 13, 639 (2023), Application(s): Chimpanzee feces,
Knockout of TSC2 in Nav1.8þ neurons predisposes to the onset of normal weight obesity: J.M. Brazill, et al.; Mol. Metab. 68, 101664 (2023),
Neural basis for fasting activation of the hypothalamic-pituitary-adrenal axis: A.M. Douglass, et al.; Nature 620, 154 (2023),
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