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文献和实验Production of a Human Antibody Library in the Phage-Display Vector pSEX81
Human monoclonal antibodies (MAbs) are more suitable than MAbs of animal origin for clinical applications because of lower hypersensitivity reactions, less formation of circulating immune complexes and lower anti-immunoglobulin responses
[欢迎下载]：Antibody Engineering： Methods and Protocols
of Human Immunoglobulin Genes 7 Antibody Humanization by CDR Grafting 8 Selection of Human Antibodies from Phage Display Libraries 9 Production of Human Single-Chain Antibodies by Ribosome Display 10 Production of Human Antibodies from Transgenic Mice
In Vitro Antibody Affinity Maturation Targeting Germline Hotspots
residues are most likely to have a major impact on antibody affinity. Here, we describe procedures for germline hotspot mutagenesis with an emphasis on strategies for randomizing hotspots with PCR and phage display, using as an example the anti-CD22
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