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抗绿色荧光蛋白抗体 亲和纯化兔多克隆 ELISA/IC/WB

GFP抗体
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  • 询价
  • 2025年07月13日
  • ELISA;Western Blotting
  • Rabbit
  • Vertebrates
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      ELISA;Western Blotting

    • 宿主：

      Rabbit

    • 库存：

      大量

    • 适应物种：

      Vertebrates

    • 抗原来源：

      0

    • 是否单克隆：

      2

    • 规格：

      50 μg

    Product Images

    Immunocytochemistry: Representative image from a previous lot.
    Confocal fluorescent analysis of RENCX constitutively expressed GFP using a previo...
    Species Reactivity Key Applications Host Format Antibody Type
    Vrt, R ELISA, IC, WB Rabbit Affinity Purified Polyclonal Antibody
    Description:
    Anti-Green Fluorescent Protein
    Background Information:
    The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
    Alternate Names:
    GFP; eGFP
    Specificity:
    AB3080P is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080P GFP antibody has been affinity purified, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.
    Key Applications:
    • ELISA
    • Immunocytochemistry
    • Western Blotting
    Application Notes:
    Western Blot:
    A 1:500-1:1000 dilution of a previous lot was used using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods.

    ELISA:
    A 1:800-1:1,000 dilution of a previous lot was used in ELISA.

    Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore's highly purified secondary antibodies in indirect detection systems.

    Immunocytochemistry:

    Optimal working dilutions and protocols must be determined by end user.
    Species Reactivity:
    • Vertebrates
    • Rat
    Species Reactivity Note:
    Most common vertebrate species tested. Reactivity with other species has not been confirmed. Expected to react with rat based on sequence homology.
    Immunogen:
    Highly purified native GFP from Aequorea victoria.
    Molecular Weight:
    Varies (GFP is 27 kDa)
    Control:
    • Cells transfected with GFP-tagged fusion vector
    • RENCX cells
    Presentation:
    Affinity purified rabbit polyclonal IgG in 10 mM Tris buffer.
    Storage Conditions:
    Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

    Handling Recommendations:
    Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
    Gene Symbol:
    • GFP
    • eGFP
    Quality Assurance:
    Routinely evaluated by immunocytochemistry on RENCX cells.

    Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot's anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.
    Antibody Category:
    Epitope Tags & General Use
    Antibody Sub-Category:
    Epitope Tags
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    Affinity Purified
    Host:
    Rabbit
    Concentration:
    1.0 mg/mL
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    Highly purified native GFP from Aequorea victoria. 详细描述见链接：http://www.millipore.com/catalogue/item/AB3080P

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    图标文献和实验
    相关实验

    • 【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体？

      Antibody均能与GFP、YFP、EYFP、CFP等突变体有交叉反应，可以用于GFP、YFP、EYFP、CFP等荧光蛋白的表达定量、定位分析和检测。EarthOx该GFP标签抗体（货号#E022030）的另一个优势是，可用于的试验类型很多，除了基本的WB、ELISA试验外，还可以用于免疫细胞化学、免疫荧光（ICC/IF）、免疫共沉淀（IP）等。该抗体的效价很高，EarthOx的推荐WB起始稀释比率为1:1000，实际应用于中还能有更优异的表现。因此，EarthOx的Anti-GFP Tag

    • 抗体的制备方法与原理-抗血清的制备

      　　有了质量好的抗原，还必须选择适当的免疫途径，才能产生质量好（特异性强和效价高）的抗体。 　　（一）用于免疫的动物 　　作免疫用的动物有哺乳类和禽类，主要为羊、马、家兔、猴、猪、豚鼠、鸡等，实验室常用者为家兔、山羊和豚鼠等。动物种类的选择主要根据抗原的生物学特性和所要获得抗血清数量，如一般制备抗r-免疫球蛋白抗血清，多用家兔和山羊，因动物反应良好，而且能够提供足够数量的血清，用于免疫的动物应适龄

    • 免疫双扩散检测抗GFP血清抗体

      [实验原理] 在溶液中的可溶性多价抗原与血清抗体相遇，当两者的比例适当时可以形成一种网状的不溶性的大分子复合物，这叫作免疫沉淀反应。当这样的抗原和相应的抗体在含有电解质的琼脂凝胶中相对扩散时，在抗原与抗体的比例适当处会形成可见的沉淀线，这叫免疫双扩散实验，是免疫沉淀反应的一种。免疫双扩散常用于动物免疫效果的检测和血清抗体效价的测定。本实验使用的粗抗原是大肠杆菌表达的GFP的抽提物，抗体是GFP免疫兔子后得到的抗血清

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