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        [欢迎下载]:Antibody Engineering: Methods and Protocols

        丁香园论坛

        4360
        内容:Antibody Engineering: Methods and Protocols
        格式:pdf
        大小:5.18M
        语种:英文
        简介:为Methods in Molecular Biology系列第248卷,由Benny K. C. Lo主编,Humana Press 2003年出版。目录如下:
        1 Internet Resources for the Antibody Engineer
        2 The Kabat Database and a Bioinformatics Example
        3 IMGT, The International ImMunoGeneTics Information System®, http://imgt.cines.fr
        4 Antibody Variable Regions: Toward a Unified Modeling Method
        5 Studying Antibody Conformations by Ultracentrifugation and Hydrodynamic Modeling
        6 PCR Cloning of Human Immunoglobulin Genes
        7 Antibody Humanization by CDR Grafting
        8 Selection of Human Antibodies from Phage Display Libraries
        9 Production of Human Single-Chain Antibodies by Ribosome Display
        10 Production of Human Antibodies from Transgenic Mice
        11 Selection of Internalizing Antibodies for Drug Delivery
        12 Engineering Multivalent Antibody Fragments for In Vivo Targeting
        13 Production of Recombinant Bispecific Antibodies
        14 Expression and Isolation of Recombinant Antibody Fragments in E. coli
        15 Expression of Recombinant Antibodies in Mammalian Cell Lines
        16 Human Antibody Production Using Insect-Cell Expression Systems
        17 Antibody Production in Transgenic Plants
        18 Directed Mutagenesis of Antibody Variable Domains
        19 Antibody Affinity Maturation by Chain Shuffling
        20 Antibody Affinity Maturation by Random Mutagenesis
        21 Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting
        22 Antibody Purification by Column Chromatography
        23 Affinity Measurement Using Surface Plasmon Resonance
        24 Kinetic Exclusion Assays to Study High-Affinity Binding Interactions in Homogeneous Solutions
        25 Characterization of Antibody–Antigen Interactions by Fluorescence Spectroscopy
        26 Antibody Epitope Mapping Using Arrays of Synthetic Peptides
        27 Mapping Antibody:Antigen Interactions by Mass Spectrometry and Bioinformatics
        28 Radiometal Labeling of Antibodies and Antibody Fragments for Imaging and Therapy
        29 Production and Characterization of Anti-Cocaine Catalytic Antibodies
        30 Recombinant Immunotoxins in the Treatment of Cancer
        31 Antibodies in Proteomics
        32 Targeting of Antibodies Using Aptamers
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