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- ZYMO RESEARCH
- 美国
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- 2026年04月21日
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- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
1年
- 英文名：
ZymoPURE II Plasmid Midiprep Kit
- 库存：
20
- 供应商：
简石生物
HIGHLIGHTS
- Perform plasmid midipreps in only 16 minutes using a simple spin-column protocol.
- Purify up to 1.2 mg of highly concentrated plasmid DNA directly from a spin-column.
- Eluted plasmid DNA is Endotoxin-free and Transfection-Ready.
DESCRIPTION
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 18 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with either a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 6 µg/µl), endotoxin-free and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
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文献和实验Midiprep preparation of Plasmid DNA
实验概要 The PureLink™ HiPure Plasmid DNA Midiprep Kit allows purification of 100–350 μg of high-quality plasmid DNA from 15–25 mL overnight E. coli cultures in ~2 hours when cloning high copy number plasmids
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
with occasional mixing. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II tightly capped when not in use.) 4. Add 250 ul ice-cold Buffer N3 and mix gently but throughly by inverting tube several times
Fastfilter Plasmid Maxi Kit Spin Protocol
Solution II, cover, and mix gently but throughly by inverting and rotating tube 10-15 times to obtain a cleared lysate. A 2 min incubation at room temperature may be necessary. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid
