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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Invalid
- 库存:
1 case
- 英文名:
35 X 10MM PS SUSP. DISH,NT, W/VENT LID
- 供应商:
西宝生物
- 规格:
LABWARE
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文献和实验Construction and Characterization of Adenovirus Vectors
) twice with 2 mL of PBS-Ad. 20. Transfer 0.7 mL of the plasmid complex suspension to each 35-mm dish of 293 cells. 21. Incubate the cells for 3 h at 37°C, 5% CO2 . 22. While the cells are incubating, prepare
[M]=A259 /15.4x103 =A259 1:2000 x0.130 adjust the final volume. Betaine monohydrate C5 H11 NO2 xH2 O; Mw=135.2g/M; (store at 4o C). Conc. Stock %(w/w) 100ml 200ml 700ml Betaine 135.2g/M 5M
mQ PBS (recipe 3) 10x pH(1x)=7.3; (store at NT or at 4o C) 1x 10x Stock 100ml 500ml KH2 PO4 2mM 20mM 136.1g/M 0.272g 1.36g Na2 HPO4 x7H2 O 10mM
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