- ￥3088.01
- Pierce
- made in USA
- 23225
- 2026年03月01日
企业认证
相关产品推荐更多 >
Sigma货号DUO92004现货Duolink原位PLA探针抗小鼠MINUS上海睿安生物19121878899
￥7353.44
Roche货号11442074001碱性磷酸酶(AP)显色底物BM紫13611631389上海睿安生物
￥1363.47
Active Motif货号52097现货Ras GTPase活性检测ELISA试剂盒13611631389上海睿安生物
￥12680
博奥龙Biodragon货号BF06001小鼠单抗Ig类/亚类鉴定用ELISA试剂盒(IgG1-IgG2a-IgG2b-IgG3-IgM-IgA)上海睿安生物13611631389
￥2900.01
R&D Systems货号MFNAS0现货Mouse IFN-alpha All Subtype Quantikine ELISA Kit上海睿安生物19121878899
￥7811.01
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
BCA蛋白定量试剂盒
- 库存：
10⁺盒
- 供应商：
上海睿安生物19121878899
- 保存条件：
Room Temperature
- 规格：
1L/Kit/盒
Pierce货号23225现货BCA蛋白定量试剂盒19121878899上海睿安生物
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.
Kits are available with or without Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.
Kit options are available that include Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
Features of the BCA Protein Assay Kit include:
- Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
- Accurate—exhibits half the protein-to-protein variation observed with dye-binding methods (Bradford)
- Compatible—unaffected by typical concentrations of most ionic and non-ionic detergents
- Short assay time—30-min incubation; much easier and four times faster than classical Lowry methods
- Wide assay range—linear working range for BSA of 20 to 2000 μg/mL
- Sensitive—detect down to 5 μg/mL with enhanced protocol
- No more serial dilutions—kit options include Dilution-Free BSA Protein Standards which eliminate the need to perform serial dilutions when generating a standard curve
How the assay works
The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid (BCA). The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.
In the second step of the color development reaction, BCA reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The complex is approximately 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.
The reaction is strongly influenced by four amino acid residues (cysteine, cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike Coomassie dye-binding methods (i.e., Bradford), the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.
•BCA 试剂A，2x500ml
•BCA 试剂B，25ml
•蛋白标准品安瓿瓶，2mg/ml，10x1ml
室温下储存
原Pierce公司创立于1950年，是蛋白质化学、免疫学和层析技术等领域的领先者，其产品独具特色、品质卓越，在世界范围内倍受赞誉。
PIERCE产品是目前进行蛋白质研究及相关研究工作的首Xuan选，其几乎涵盖了蛋白质化学的所有研究领域，包括蛋白质的抽提、纯化、电泳、定量、标记、检测、结构研究、功能研究以及相互作用研究等等，其中BCA总蛋白定量试剂已经成为目前最为流行、应用最为广泛的总蛋白定量试剂。另外，PIERCE的化学发光技术处于世界领先地位，许多著名的化学发光试剂盒生产厂商均采用PIERCE的底物作为原料；PIERCE全面而独到的抗体制备、纯化和修饰技术更为科研工作中遇到的各种挑战提供了完整的解决方案。
订购信息
| 货号 | 产品名称 | 规格 | 目录价 |
| 23225 | Pierce 货号23225 BCA蛋白定量试剂盒 | Kit | 2263 |
| 23227 | Pierce 货号23227 BCA蛋白定量试剂盒 | Kit | 1396 |
| 32209 | Pierce 货号32209 Pierce ECL Western Blotting Substrate | 250 mL Kit | 1635 |
| 34077 | Pierce 货号34077 SuperSignal West Pico 化学发光底物试剂盒（100 mL） | 100 mL | 1031 |
| 34080 | Pierce 货号34080 SuperSignal West Pico 化学发光底物试剂盒（500 mL） | 500mL kit | 2979 |
| 34095 | Pierce 货号34095 SuperSignal West Femto 化学发光底物试剂盒 | 100mL kit | 4610 |
| 34079 | Pierce 货号34079 SuperSignal West Pico Trial Kit | 50mL kit | 649 |
睿安公司专业技术服务
荧光定量PCR、甲基化检测、文库构建、原核和真核蛋白表达、高通量测序、抗体定制服务、WB检测、IHC检测。
睿安公司代理及优势品牌：
免疫学：Abgent、CST、R&D、Abcam、BD、Epitomics、Santa cruz、Jackson
耗材类： Ammex、Fisher、Bioplastics、Axygen、Corning、Greiner，Nunc-Nalgene
分子和细胞：Fermentas、hyclone-pierce、Qiagen、Bio-Rad
综合类： Sigma、 MERCK、Millipore、Roche、Invitrogen
自主品牌：Rainbio 生化类：Sigma-Vetec 、 MP
肥胖代谢类：Biovendor、ALPCO
表观遗传：Zymo ACTIVE MOTIF 神经和信号传导：Tocris
其他类： Biovision、Biocolor、Covance、Cytoskeleton、ebioscience、GE、Genetex、Invivogen、Omega、
Proteintech 、Socorex 、Vector
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验Pierce货号23225现货BCA蛋白定量试剂盒19121878899上海睿安生物
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.
Kits are available with or without Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.
Kit options are available that include Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
Features of the BCA Protein Assay Kit include:
- Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
- Accurate—exhibits half the protein-to-protein variation observed with dye-binding methods (Bradford)
- Compatible—unaffected by typical concentrations of most ionic and non-ionic detergents
- Short assay time—30-min incubation; much easier and four times faster than classical Lowry methods
- Wide assay range—linear working range for BSA of 20 to 2000 μg/mL
- Sensitive—detect down to 5 μg/mL with enhanced protocol
- No more serial dilutions—kit options include Dilution-Free BSA Protein Standards which eliminate the need to perform serial dilutions when generating a standard curve
How the assay works
The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid (BCA). The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.
In the second step of the color development reaction, BCA reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The complex is approximately 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.
The reaction is strongly influenced by four amino acid residues (cysteine, cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike Coomassie dye-binding methods (i.e., Bradford), the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.
•BCA 试剂A，2x500ml
•BCA 试剂B，25ml
•蛋白标准品安瓿瓶，2mg/ml，10x1ml
室温下储存
原Pierce公司创立于1950年，是蛋白质化学、免疫学和层析技术等领域的领先者，其产品独具特色、品质卓越，在世界范围内倍受赞誉。
PIERCE产品是目前进行蛋白质研究及相关研究工作的首Xuan选，其几乎涵盖了蛋白质化学的所有研究领域，包括蛋白质的抽提、纯化、电泳、定量、标记、检测、结构研究、功能研究以及相互作用研究等等，其中BCA总蛋白定量试剂已经成为目前最为流行、应用最为广泛的总蛋白定量试剂。另外，PIERCE的化学发光技术处于世界领先地位，许多著名的化学发光试剂盒生产厂商均采用PIERCE的底物作为原料；PIERCE全面而独到的抗体制备、纯化和修饰技术更为科研工作中遇到的各种挑战提供了完整的解决方案。
订购信息
| 货号 | 产品名称 | 规格 | 目录价 |
| 23225 | Pierce 货号23225 BCA蛋白定量试剂盒 | Kit | 2263 |
| 23227 | Pierce 货号23227 BCA蛋白定量试剂盒 | Kit | 1396 |
| 32209 | Pierce 货号32209 Pierce ECL Western Blotting Substrate | 250 mL Kit | 1635 |
| 34077 | Pierce 货号34077 SuperSignal West Pico 化学发光底物试剂盒（100 mL） | 100 mL | 1031 |
| 34080 | Pierce 货号34080 SuperSignal West Pico 化学发光底物试剂盒（500 mL） | 500mL kit | 2979 |
| 34095 | Pierce 货号34095 SuperSignal West Femto 化学发光底物试剂盒 | 100mL kit | 4610 |
| 34079 | Pierce 货号34079 SuperSignal West Pico Trial Kit | 50mL kit | 649 |
睿安公司专业技术服务
荧光定量PCR、甲基化检测、文库构建、原核和真核蛋白表达、高通量测序、抗体定制服务、WB检测、IHC检测。
睿安公司代理及优势品牌：
免疫学：Abgent、CST、R&D、Abcam、BD、Epitomics、Santa cruz、Jackson
耗材类： Ammex、Fisher、Bioplastics、Axygen、Corning、Greiner，Nunc-Nalgene
分子和细胞：Fermentas、hyclone-pierce、Qiagen、Bio-Rad
综合类： Sigma、 MERCK、Millipore、Roche、Invitrogen
自主品牌：Rainbio 生化类：Sigma-Vetec 、 MP
肥胖代谢类：Biovendor、ALPCO
表观遗传：Zymo ACTIVE MOTIF 神经和信号传导：Tocris
其他类： Biovision、Biocolor、Covance、Cytoskeleton、ebioscience、GE、Genetex、Invivogen、Omega、
Proteintech 、Socorex 、Vector
