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- 文献和实验
- 技术资料
- 英文名:
Platinum® Taq DNA Polymerase, High Fidelity
- 供应商:
研卉生物
- 规格:
100 reactions
11304-011
描述
Platinum® Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments where high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´-5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase (Table 1). PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology.• Fidelity – Greater than six times higher fidelity than Taq DNA polymerase
• Amplicon Size – Amplification of fragments up to 15 kb (Figure 1)
• Convenience – Room temperature reaction assembly
• Applications – Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR
Unit Definition: One unit of Platinum® Taq DNA Polymerase High Fidelity is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min. at 74°C.
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文献和实验SuperScript III One-Step RT-PCR System with Platinum® Taq High Fidelity
. Because SuperScript™ III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. Platinum® Taq DNA Polymerase High Fidelity is an enzyme mixture composed of recombinant Taq DNA polymerase
实验步骤 1. Measure out the amount of Taq DNA polymerase needed. When using a reaction cocktail, determine the total number of units in the mix. 2. Add an amount of “equivalent units” of Platinum® Taq
使用 Platinum® Taq DNA 聚合酶 (Platinum® Taq DNA Polymerase),对靶序列进行定性验证
实验步骤 The procedure on the following page is suggested as a guideline and starting point when using Platinum® Taq DNA Polymerase in any PCR amplification. Optimal reaction conditions (incubation times and temperatures
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