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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
10 mM dNTP Mix
- 库存:
大量
- 规格:
100 µl
详细说明
常用规格
| Regulatory Statement: | For Research Use Only. Not for any animal or human therapeutic or diagnostic use. |
| Concentration (molarity): | 10 mM |
| Product Size: | 100 µl |
内容和储存
These nucleotides are supplied as convenient, ready-to-use solutions at a concentration of 10 mM in Tris-HCl (pH 7.5). The 10 mM dNTP Mix contains all four of the dNTPs, each at a final concentration of 10 mM. Store at -20°C. Guaranteed stable for 6 months when properly stored.
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文献和实验用ReadyMixTM PCR Reaction Mix进行拟南芥SSLP
用ReadyMix TM PCR Reaction Mix 进行拟南芥SSLP (32 个样品+3 个对照) 一、PCR 采用SIGMA REDTaq® ReadyMixTM PCR Reaction Mix提供的 试剂 (包括20 mM Tris-HCl, pH 8.3, 100 mM KCl, 3 mM MgCl2, 0.002% gelatin, 0.4 M dNTP
Protocol for isolating DNA from embryo yolk sacs, or toes, or 2 mm tail for PCR
HCl. 5. Vortex. The DNA is now in a 20 mM Tris/0.1 mM EDTA at pH 8.1 with about 12.5 mM Na++ ions. That's pretty close to the 10 mM Tris/ 0.1 mM EDTA / pH 8 solution used to store DNA. 6. Use 1 µl for PCR 7. (May need to microfuge for 6 minutes
7.5,小量分装, -20℃冰冻保存。多次冻融会使dNTP降解。在PCR反应中,dNTP应为50~200umol/L,尤其是注意4种dNTP的浓度要相等( 等摩尔配制),如其中任何一种浓度不同于其它几种时(偏高或偏低),就会引起错配。浓度过低又会降低PCR产物的产量。dNTP能与Mg2+结合,使游离的Mg2+浓度降低。 dNTP储存液:dNTP溶于pH为7.0的NaOH贮存液中,最初的贮存液可稀释到10mol/L,分装后存放在-20℃冰箱中。dNTP使用浓度在20~200 μmol/L之间










