使用 Platinum® Taq DNA 聚合酶 (Platinum® Taq DNA Polymerase)，对靶序列进行定性验证
互联网
1263
实验步骤
Template DNA ≥ 1 µl (as required)
Platinum® Taq DNA Polymerase 0.2 µl
Autoclaved, distilled water to 50 µl2. Mix contents of the tubes and overlay with 50 µl of mineral or silicone oil, if necessary.
3. Cap the tubes and centrifuge briefly to collect the contents.
5. Perform 25-35 cycles of PCR amplification as follows:
6. Maintain the reaction at 4°C after cycling. The samples can be stored at -20°C until use.
