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文献和实验The ribonuclease protection assay (RPA)
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant RNA polymerases from the bacteriophages SP6, T
Quantitation of RNA by Ribonuclease Protection Assay
A robust ribonuclease protection assay is described here. In brief, total cellular RNA, carrier yeast transfer RNA, and 32 P-labeled antisense riboprobes, (one or more designed to detect the RNA species being studied and another to detect
The RNase protection assay is based on the resistance of RNA:RNA hybrids to single-strand specific RNases, after annealing to a complementary 32 P-labeled probe in solution. It can be used to map the ends of RNA molecules or exon-intron
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