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文献和实验The ribonuclease protection assay (RPA)
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant RNA polymerases from the bacteriophages SP6, T
Quantitation of RNA by Ribonuclease Protection Assay
A robust ribonuclease protection assay is described here. In brief, total cellular RNA, carrier yeast transfer RNA, and 32 P-labeled antisense riboprobes, (one or more designed to detect the RNA species being studied and another to detect
RNase Protection Assay--RNA酶保护
For quantitating RNA levels from either polyA+ selected RNA or crude RNA preps. Solutions 10X Hybridization Buffer 0.4 M PIPES 12.1 g PIPES (Sigma #P6757) 4.0 M NaCl 23.4 g NaCl 10 mM EDTA 2.0 ml 500 mM EDTA pH to 6.7 and bring up to 100 ml
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