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- 详细信息
- 文献和实验
- 技术资料
- 服务名称:
Cytokine Production DNA Methylation PCR Array
- 提供商:
SAB
B Cell Function Regulators: BCL10, BCL3, INHA, INHBA, STAT5A.
Transcriptional Regulators: BCL10, BCL3, FOXP3, GATA3, IRF1, SMAD3, STAT5A.
Translational Regulators: BCL3, IGF2BP2.
Environment & Intracellular Stimuli Responses: BCL10, BCL3, ELA2, FOXP3, GATA3, HMOX1, IL12A, INHA, INHBA, LTB, MALT1, MYD88, NOD1, SMAD3, SOD1, STAT5A, TLR2.
Cytokine Production Signaling Molecules: BCL10, BCL3, FOXP3, HMOX1, INHA, INHBA, LTB, MALT1, MAP3K7, MYD88, NOD1, SMAD3, SOD1, STAT5A, TLR2, TRAF2, TRAF6.
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文献和实验和PCR反应体系,适应PCR芯片的要求。 RT2ProfilerTM PCR芯片引物的特点 通过计算机分析和实验验证,引物达到以下要求: 1、特异性:通过BLAST等比对方法,检测确认引物在相应物种(人,小鼠或大鼠)全基因组中的高度特异性,有效避免非特异性产物产生。并通过实验证明引物不会扩增E.coli基因组,后者是Taq DNA多聚酶的常见污染源。 2、均一性:所使用的引物的CG含量,解链温度(Tm),以及其他一些化学的和物理的特性都相似,以保证在相同的PCR条件(尤其是相同退火
Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization
DNA methylation is an important epigenetic modification that regulates development and plays a role in the pathophysiology of many diseases. It is dynamically changed during germline development. Methylated DNA immunoprecipitation (MeDIP
of techniques have been developed. Most early studies used methylation sensitive restriction enzymes to digest DNA followed by Southern detection or PCR amplification. Recently, bisulfite reaction based methods have become very popular such as methylation
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