Recombinant Human IL-36 alpha

Recombinant Human IL-36 alpha

Recombinant Human IL-36 alpha (CR61)

产品说明(Description)

Recombinant Human Interleukin-36 Alpha is produced by our E.coli expression system and the target gene encoding Lys6-Phe158 is expressed.

Accession #: Q9UHA7

Known as: IL-36 alpha; IL-36α; Interleukin-36 Alpha; FIL1 Epsilon; Interleukin-1 Epsilon; IL-1 Epsilon; Interleukin-1 Family Member 6; IL-1F6; IL36A; FIL1E; IL1E; IL1F6

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 1 mM EDTA, 0.02% Tween 20, pH 8.0.

质量控制(Quality Control)

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity: Measured in a cell proliferation assay using A-431 Human epithelial carcinoma cells. The ED50 for this effect is 12.0 ng/ml.

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景(Background)

Human Interleukin-36α (IL-36α) is a secreted cytokine that belongs to the Interleukin 1 cytokine family. IL-36α is expressed in the immune system and the fetal brain, but not in other tissues or multiple hematopoietic cell lines. IL-36α is the only IL-1 family member found to be expressed on T-cells. IL-36α and IL-1F8 are involved in the regulation of adipose tissue gene expression. Importantly, IL-36α inhibits PPARγ expression, which may lead to a reduction in adipocyte differentiation suggesting metabolic effects of this cytokine. IL-36α, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFκB and MAPK in an IL-1Rrp2 dependent manner. This suggest that IL-36α may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

电泳(SDS-PAGE)

FOR RESEARCH USE ONLY仅限科学研究
20年前，白介素36（IL-36）作为IL-1超家族的成员被发现。现有的四个亚型已多次重命名，以前被称为IL-1F6、IL-1F8、IL-1F9和IL-1F5，十年前，由于其功能被确定，因此它们被命名为IL-36α、IL-36β、IL-36γ和IL-36受体拮抗剂（IL-36 receptor antagonist, IL-36 Ra）。IL-36受体（IL-36 receptor, IL-36R）也被称为IL-1Rrp2或者是IL1RL2，最终被确认为IL-1R6，它的配体包含IL-36家族的所有成员：IL-36α，IL-36β，IL-36γ和IL-36 Ra。此外，IL-38也可以结合此受体。