- ¥1560
- SignaGen
- 美国
- Catalog #: SL100489-COS
- 2026年01月04日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
一年
- 英文名：
GenJet™ In Vitro DNA Tranfection Reagent for COS Cells
- 库存：
现货 大量
- 供应商：
济南美中清水湾生物科技有限公司
- 保存条件：
4°C
GenJet™ DNA In Vitro Tranfection Reagent for COS Cells is pre-optimized for COS cell transfection. COS is a cell line often used by biologists when studying the monkey virus SV40. It is also often used to transfect cells to produce recombinant proteins for molecular biology, biochemistry, and cell biology experiments. Two forms of COS cell lines commonly used are COS-1 and COS-7. The COS cell line was obtained by immortalizing a cell line derived from kidney cells of the African green monkey with a version of the SV40 genome that can produce large T antigen but has a defect in genomic replication.
Refer to the following optimal transfection conditions for maximal transfection efficiency on both COS-1 and COS-7 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium 5 Hours After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
~90% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes No 48 hours EGFP pEGFP-N3 (CMV promoter) 88% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Two Pictures Showing Transfection Efficiency of GenJet™ Reagent on COS-1 (upper panel) and COS-7 (lower panel) Cells Respectively. COS-1 cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection
Data Sheet
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文献和实验COS-1 cells were created by transforming an established line of monkey epithelial cells, CV-1, with a defective mutant of SV40 (1 ). The SV40 mutant used carried a small deletion within the origin of replication and, although this construct
Transient Transfection of Cos-1 Cells
Transient Transfection of Cos-1 Cells Note: This protocol has been optimized for Cos-1 cells. Successful transfection of each cell type requires optimization of the basic protocol. Variables to consider for optimization include
Note: This protocol has been optimized for Cos-1 cells. Successful transfection of each cell type requires optimization of the basic protocol. Variables to consider for optimization include, but are not limited to, cell density, transfection
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