- ¥1560
- SignaGen
- 美国
- SL100489-D407
- 2026年04月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
一年
- 英文名：
GenJet™ In Vitro DNA Tranfection Reagent for D 407 Cells
- 库存：
现货 大量
- 供应商：
济南美中清水湾生物科技有限公司
- 保存条件：
4°C
- 规格：
毫升/支
GenJet™ DNA In Vitro Tranfection Reagent for D 407 is pre-optimized for transfecting D 407, retinal pigment epithelial cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on D 407 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 wellplates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, 18~24 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter) 70% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
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文献和实验相关专题 总有一种转染方法适合你 近日，罗氏公司新推出了两款高性能的DNA转染试剂，为其细胞分析产品线再添新军。这两款名为X-tremeGENE 9和X-tremeGENE HP的转染试剂沿用了X-tremeGENE siRNA 转染试剂的前缀，应该与大名鼎鼎的Fugene 6不属于一类。据罗氏介绍，这两个产品是多组分的独特试剂，并非脂质体。它们能够提供高的转染效率、重复性和细胞存活率。 这两款新产品都融合了罗氏二十
, D., Accornero, N., Lavoie, B., Shenoy, S.M., Blanchard, J.M., Singer, R.H., and Bertrand E. 2003. Single mRNA molecules demonstrate probabilistic movement in living mammalian cells. Curr. Biol. 13:161‐167.
and expression of foreign genes in mammalian cells. BioTechniques 29, 314-318, 320-312, 324 passim.6. Mangeot, P.E., Negre, D., Dubois, B., Winter, A.J., Leissner, P., Mehtali, M., Kaiserlian, D., Cosset, F.L., and Darlix, J.L. （2000）。 Development of minimal
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