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100 µg
蛋白名称:MD2/LY96蛋白, MD2/LY96 protein
蛋白构建:A DNA sequence encoding the mouse LY96 (Q9JHF9) (Met1-Asn160) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 19
蛋白分子量:The recombinant mouse LY96/Fc is a disulfide-linked homodimer. The reduced monomer comprises 383 amino acids and has a predicted molecular mass of 43.4 kDa. The apparent molecular mass of the protein is approximately 47 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:Q9JHF9
蛋白氨基酸序列:Met1-Asn160
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
注意的是,两种配体的洗脱和再生buffer稍有差别,使用的时候还需注意! 系统特点 高达 pM 范围的亲和力(KD up to 6.2 x 10-11 M) 纯化树脂材质稳定 小标签 (8-28 aa)无需移除,不影响蛋白折叠和功能 极高的蛋白纯度(纯度>95%) 应用于多种目标蛋白检测系统:Western blot, ELISA, 免疫荧光, FACS等 兼容多种缓冲条件:高盐,洗涤剂,金属离子,螯合剂,还原剂 适用于多种蛋白的纯化 Twin-Strep-tag®标签蛋白固定在界面上时 (SPR
CUT&Tag(Cleavage Under Targets and Tagmentation)【1】是一种研究蛋白质-DNA 相互作用的新技术。与传统的染色质免疫沉淀(Chromatin Immunoprecipitation, ChIP)相比,CUT&Tag 具有信噪比高、可重复性好、实验周期短(1 天实现从细胞到文库构建)、细胞投入量低等显著优势。该方法一经问世,便受到广大科研工作者的青睐。 在过去的一年时间里,科研工作者在动物、植物细胞中成功应用 CUT&Tag
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