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- 询价记录
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
EpiGnome™ Methyl-Seq Kit
- 规格：
12 次反应
• 预制文库亚硫酸盐转化
• 高度多样性文库
• Uniform CpG, CHG, and CHH coverage
• 全步骤仅需一天
• 仅需50纳克DNA
| Application:
Epicentre's methyl-seq kit produces whole genome bisulfite sequencing libraries from only 50 ng of genomic DNA. EpiGnome™ follows a unique "post-bisulfite conversion" library construction method which yields highly diverse libraries with uniform coverage. |
|
- Pre-library bisulfite conversion
- Highly diverse libraries
- Uniform CpG, CHG, and CHH coverage
- 1-day procedure
- Only 50 ng into bisulfite-conversion
 |
Figure 1 (click to enlarge). Bisulfite treated ssDNA is random primed using a polymerase able to read uracil nucleotides to synthesize DNA strands containing a specific sequence tag. The 3´-ends of the newly synthesized DNA strands are then selectively tagged with a second specific sequence tag using a patented procedure resulting in di-tagged DNA molecules with known sequence tags at its 5´ and 3´ ends. The di-tagged DNA is enriched in PCR resulting in dsDNA with the appropriate sequences required for sequencing on any Illumina platform. |
 |
Figure 2 (click to enlarge). Example of CpG methylation pattern across region of chr1 indicates high CpG methylation (red) and regions of low CpG methylation (blue) from 50 ng input of GM12878 lymphoblastoid gDNA treated with bisulfite. For the hypermetylated (CpG methylase) HeLa gDNA, majority of CpGs detected were clearly methylated. The IGV plots shown are based on forward reads for each sample. |
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文献和实验including the lower amount of input DNA required, an expanded dynamic range and compatibility with sample multiplexing. Here we describe a detailed protocol for profiling histone modification in the Arabidopsis thaliana genome with ChIP-seq using the SOLiD™
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epignome-methyl-seq-kit.pdf 附 (下载 79 次)
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