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文献和实验Chromosomal DNA Extraction from Gram-positive Bacteria
This procedure was originally developed for Listeria monocytogenes but has worked well with other Gram+ bacteria we've tried. 1.Pellet cells from 10 ml overnight cultures in BHI or LB and wash in 5 ml of 0.1X SSC. 2.Resuspend in 1 ml 10 mM Tris
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
Antibody Production by the Gram-Positive Bacterium Bacillus megaterium
but they showed only a limited capacity to secrete antibody fragments into the medium – a prerequisite for easy downstream processing. Gram-positive bacteria are known to efficiently secrete recombinant proteins into the medium. Recently, we demonstrated
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