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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
qPCR hRef.cDNA,oligo(dT)primed
- 供应商:
北京智杰方远
- 保存条件:
-20度
- 规格:
25 次
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文献和实验and redissolve in 20 µl H 2ODEPC measure RNA concentration and check integrity on a denaturing gel II. cDNA synthesis for each RNA set up four reactions (one tube for each degenerate anchored oligo(dT) primer set - T 12MA,T12 MC
Differential Display Technical notes
Optimal arbitrary primer length for Differential Display The concept of differential display is to use a limited number of short arbitrary primers in combination with the anchored oligo-dT primers to systematically
Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer
to the prehybridization and hybridization reactions at 1 μg/ml to prevent nonspecific hybridization between the oligo(dT) tails of the cDNA and oligo(dA) tracts in the genomic DNA.
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