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QIAGEN Genomic-tip 500/G
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文献和实验in the Beckman. Promptly pour supernatant into another round-bottom 13 ml polycarbonate tube and re-centrifuge at 20,000 x g for 15 min. While the centrifugation is happening, equilibrate a Qiagen-tip 100 (see next step). 7. To equilibrate the tip
but thoroughly, immediately after addition of Buffer N3. The solution should become cloudy. Centrifuge for 10 min at 13,000 rpm (~17,900 x g) in a table-top microcentrifuge. A compact white pellet will form. Apply the supernatants from step
Qiagen MIDI protocol(QIAGEN plasmid Midi试剂盒)
and re-centrifuge at 20,000 x g for 15 min. While the centrifugation is happening, equilibrate a Qiagen-tip 100 (see next step). 7. To equilibrate the tip, put it in a plastic collar and put this assembly into the top of a 50-ml conical polypropylene tube. Add
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