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Oligotex mRNA Mini Kit
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文献和实验application. 3. RNA Storage Store the purified RNA on ice for immediate use. For long–term storage, keep the purified RNA at –80°C. Perform DNase I treatment after purification (refer to the PureLink™ RNA Mini Kit manual) to assure highly
of containing most of the necessary reagents pre-packaged in RNase-free quality (e.g. polyATtract from Promega or Oligotex mRNA kit from Qiagen work well for Neurospora, Table 1)。 Depending on the kit size, this allows isolation of poly(A)-RNA from 0.25 to 5 mg
【共享】经验:The RNeasy Mini Kit from Qiagen
The RNeasy Mini Kit from Qiagen is designed for RNA extraction, although the basic format is similar to other Qiagen products designed to purify nucleic acids: ion exchange columns specifically bind RNA which is purified by wash steps
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