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Worktable mount
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文献和实验image of a whole mount from a 4 wk old virgin Protocol Spread tissue on glass slide Fix in Carnoy's fixative for 2 to 4 hours at r.t. Wash in 70 % EtOH for 15 min Change gradually to distilled water
Immunofluorescence- Whole Mount
Objective: Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically, a primary antibody binds specifically to an antigen (e.g. Luciferase); then a secondary antibody conjugated to a flourochrome
The first step in almost every investigation of skeletal phenotypes is analysis of whole-mount skeletal preparations. Whole-mount skeletal staining permits evaluation of the shapes and sizes of skeletal elements in their appropriate locations
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