实验材料:胚胎(13 天)(20 mg),肾脏(15 mg),肝脏(10 mg),脾(15 mg),胸腺(10 mg),肺(20 mg)
实验方法:应用TIANGEN RNAprep Pure 动物组织总RNA 提取试剂盒(DP431)提取大鼠不同组织总RNA
实验结果:以上为提取样本的总RNA 电泳图。
提取后洗脱体积100 μl,RNA 上样量2-4 μl。
M:TIANGEN DNA Marker III;
Lane 1-2:胚胎(13 天) Lane 3:肾脏
Lane 4-6:肝脏 Lane 7:脾
Lane 8:胸腺 Lane 3:肺
琼脂糖凝胶浓度为1%,6v/cm 电泳30 min。
大鼠不同组织提取得率
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