HRP Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[M2]产品图
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HRP Anti-DDDDK tag (Binds to F

LAG® tag sequence)抗体[M2]
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  • ¥3678
  • Abcam
  • ab49763
  • 2026年08月31日
  • Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk, flag, flag tag

    • 宿主

      Mouse

    • 供应商

      广州市左克生物

    • 规格

      100µg

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    产品简介:

    品牌:abcam

    货号:ab49763

    产品名称:HRP Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[M2]

    产品规格:100µg

    应用:WB, ELISA

    种属反应性:

    宿主种属:Mouse

    产品概述HTML:

    隐藏别名:DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk, flag, flag tag

    关键信息HTML:

    关键信息

    宿主种属

    Mouse

    克隆

    Monoclonal

    克隆号

    M2

    亚型

    IgG1

    偶联物

    HRP

    激发波长/发射波长
    不含载体蛋白

    No

    应用

    WB, ELISA

    applications

    反应性数据HTML:

    反应性数据

    { "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "

    ", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "

    " } } }

    产品用途HTML:

    产品实验方案

    For this product, it's our understanding that no specific protocols are required. You can visit:
    • Visit the General protocols
    • Visit the Troubleshooting

    产品详情HTML:

    产品详情

    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ELISA, WB.

    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) has been cited over 88 times in peer reviewed journals and is trusted by the scientific community.

    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) has 8 independent reviews from customers.

    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) specifically detects DDDDK tag (Binds to FLAG® tag sequence) (UniProt ID: ; Molecular weight: 1kDa) and is sold in a 100 ug selling size.

    This product was changed from ascites to tissue culture supernatant on 3rd December 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    性能和储存信息HTML:

    性能和储存信息

    形式
    Lyophilized
    复溶
    Reconstitute to 1.0 ml with distilled deionized water. Vortex gently and allow the vial to sit on ice for 20 minutes. After reconstitution, store the product at 4°C in the dark and use within 6 months.
    纯化工艺
    Affinity purification Protein A
    存储溶液
    pH: 7.4 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 1.5% BSA, 0.58% Sodium chloride
    运输条件
    Blue Ice
    推荐的短期储存条件
    +4°C
    推荐的长期储存条件
    +4°C
    储存信息
    Store in the dark

    靶点信息HTML:

    靶点信息

    See full target information DDDDK tag (Binds to FLAG® tag sequence)

    文献1:Nature communications 16:8682 PubMed41027936 2025 Synthetic protein degradation circuits using programmable cleavage and ligation by Sortase A. Applications Unspecified application Species Unspecified reactive species Hopen K Yang,Pragati K Muthukumar,Wilfred Chen

    文献2:Nature communications 16:8623 PubMed41022713 2025

    文献3:Structural insights into chemoresistance mutants of BCL-2 and their targeting by stapled BAD BH3 helices.

    产品细节图片1

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    图标文献和实验
    该产品被引用文献
    官网有文献
    相关实验
    • Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions

      followed by elution with desthiobiotin. (2) Purification by the FLAGtag moiety: binding to antiFLAG M2 affinity matrix followed by elution with FLAG peptide. Abbreviations: sp., specific interactors (shown as gray circles); n.sp., nonspecific proteins

    • 在免疫沉淀IP免疫荧光IF如何正确的选择Flag标签抗体

      蛋白的功能、分泌性以及转运发生改变。由于它的亲水性特点,FLAG标签倾向于定位在融合蛋白表面,因此更易与抗体结合以及被肠激酶酶解。 由于Flag标签可位于蛋白质的C端或N端,这一系统已用于各种细胞类型,包括细菌、酵母和哺乳细胞。由于Flag标签系统的纯化条件是非变性的,因此可以纯化所有有活性的融合蛋白。EarthOx的Anti-Flag Tag Monoclonal Antibody (货号#E022060 )可以用于检测和Flag标签融合表达蛋白的表达、细胞内定位,以及纯化、定性或定量检测Flag

    • 【共享】在免疫沉淀IP:免疫荧光IF实验中关于Flag抗体的应用

      蛋白表面,因此更易与抗体结合以及被肠激酶酶解。 由于Flag标签可位于蛋白质的C端或N端,这一系统已用于各种细胞类型,包括细菌、酵母和哺乳细胞。由于Flag标签系统的纯化条件是非变性的,因此可以纯化所有有活性的融合蛋白。EarthOx的Anti-Flag Tag Monoclonal Antibody(货号#E022060)可以用于检测和Flag标签融合表达蛋白的表达、细胞内定位,以及纯化、定性或定量检测Flag融合表达蛋白等。比如在免疫荧光IF的实验里,同时配合EarthOx的Dylight

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    文献支持
    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[M2]
    ¥3678