Anti-TOMM20 抗体 [4F3] - BSA and

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

4F3

亚型

IgG1

轻链类型

kappa

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

WB, IHC-P, Flow Cyt, ICC/IF, IP

applications

免疫原

Recombinant Full Length Protein corresponding to Human TOMM20.

Q15388

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "

", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "

", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "

", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "

" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "

", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "

", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

• Visit the General protocols
• Visit the Troubleshooting

产品详情

Product Specifications
Anti-TOMM20 antibody [4F3] - BSA and Azide free (ab56783) is a mouse monoclonal antibody provided in a carrier-free, conjugation ready format.
ab56783 is validated in Flow Cyt, ICC/IF, IHC-P, IP and WB in human, mouse and rat samples.
Carrier-free antibodies are compatible with our best-in-class range of Lightning-Link® conjugation kits.
Anti-TOMM20 antibody [4F3] - BSA and Azide free (ab56783) specifically detects TOMM20 (UniProt ID: Q15388; Molecular weight: 16kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Antibody clone 4F3 has been cited over 241 times in peer reviewed journals and is trusted by the scientific community.
Anti-TOMM20 antibody [4F3] - BSA and Azide free (ab56783) has 22 independent reviews from customers.

This product was changed from ascites to tissue culture supernatant on 22 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

推荐的短期储存时间

1-2 weeks

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

靶点信息

Central receptor component of the translocase of the outer membrane of mitochondria (TOM) complex essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins (PubMed : 40080546). Together with TOMM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (PubMed : 18331822). The TOM complex associates with the ion channel VDAC2 and PINK1 kinase at depolarized mitochondria, this interaction stabilizes PINK1 at the outer mitochondrial membrane and triggers downstream mitophagy by the recruitment of the E3 ubiquitin ligase PRKN (PubMed : 40080546). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity).

See full target information TOMM20