Anti-160 kD Neurofilament Medium 抗体 [NF-09] - Neuronal Marker产品图

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Anti-160 kD Neurofilament Medi

um 抗体 [NF-09] - Neuronal Marker

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价格￥3642

品牌Abcam
货号ab7794
更新日期2026年08月31日

宿主Mouse
适应物种Mouse, Rat, Human, Pig, Cow, Cat

广州市左克生物科技发展有限公司

2 年钻石会员

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技术资料

抗体名：
NEF3, NFM, NEFM, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein
宿主：
Mouse
适应物种：
Mouse, Rat, Human, Pig, Cow, Cat
供应商：
广州市左克生物
规格：
100µg

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产品简介:

品牌：abcam

货号：ab7794

产品名称：Anti-160 kD Neurofilament Medium 抗体 [NF-09] - Neuronal Marker

产品规格：100µg

应用：IHC (PFA fixed), WB, ICC/IF, IHC-Fr, ELISA, IHC-P

种属反应性：Mouse, Rat, Human, Pig, Cow, Cat

宿主种属：Mouse

产品概述HTML：

Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) is a mouse monoclonal antibody detecting 160 kD Neurofilament Medium in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Cat, Cow, Human, Mouse, Pig, Rat.

- KO validated for confirmed specificity
- Over 50 publications
- Trusted since 2002

查看别名

NEF3, NFM, NEFM, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

隐藏别名：NEF3, NFM, NEFM, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

关键信息HTML：

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

NF-09

亚型

IgG2a

轻链类型

unknown

不含载体蛋白

No

反应种属

Mouse, Rat, Human, Pig, Cow, Cat

应用

IHC (PFA fixed), WB, ICC/IF, IHC-Fr, ELISA, IHC-P

applications

特异性

This antibody reacts with both phosphorylated and non-phosphorylated forms of medium neurofilament protein (160 kDa) of various species.

反应性数据HTML：

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCPFAfixed" : {"fullname" : "Immunohistochemistry (PFA fixed)", "shortname":"IHC (PFA fixed)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "

", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "

", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "

", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "

", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "

" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "

", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "

", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "

", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "

", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "

" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "

", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "

", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "

", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "

", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "

" }, "Cat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "

", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "

", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "

", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "

" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "

", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "

", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "

", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "

" }, "Mammals": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "predicted", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Pig": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "

", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "

", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "

", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "

", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "

" } } }

产品用途HTML：

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

产品详情HTML：

产品详情

What is this antibody validated in?
Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Cat, Cow, Human, Mouse, Pig, Rat samples.

Trusted by the scientific community
Anti-160 kD Neurofilament Medium [NF-09] - Neuronal Marker (ab7794) was first used in a scientific publication in 2002 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-160 kD Neurofilament Medium [NF-09] - Neuronal Marker (ab7794) has over 5 independent reviews from customers.

Specificity confirmed
The specificity of Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) has been confirmed by Western blot testing in NEFM Knockout HEK-293 cells.

性能和储存信息HTML：

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

运输条件

Blue Ice

推荐的短期储存时间

1-2 weeks

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

靶点信息HTML：

靶点信息

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFM

文献1：Nature 646:1232-1242 PubMed40931074 2025

文献2：Neuronal activity-dependent mechanisms of small cell lung cancer pathogenesis.

文献3：Nature neuroscience 28:1146-1159 PubMed40442499 2025

产品细节图片1

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文献和实验

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相关实验

（共享）免疫组化常用标记抗体
) Distinction between carcinoma cells (calretinin negative) and reactive mesothelial cells (calretinin positive).d) Confirmation of mesothelial nature of cells in tissue specimens (such as entrapped cells and adenomatoid tumor)e) Marker of neuronal
（共享）免疫单词缩写及翻译
acetylcholine receptor 烟碱型乙酰胆碱受体 NADH nicotinamide adenine dinucleotide diaphorase 尼克酰胺腺嘌呤二核苷酸黄递酶 NANC non-adrenergic-non-cholinergic 非肾上腺素能非胆碱能 NE norepinephrine 去甲肾上腺素 NF neurofilament 神经丝 NK natural killer 自然杀伤细胞 NNE non-neuronal
[资源]【整理】蛋白版05年12月无人应助贴
,(我用的是预染的MARKER),我没用丽春红染色,用BSA封闭3小时(室温)直接加的一抗4度过夜,然后加的二抗3小时,然后显色,条带出现的好象有点靠上啊,(而且出现好多模糊的蛋白的条带,是没封闭好吧?) 跟MARKER比我也不知道到底在哪,caspase3是32KD,活化成17,12KD 两个亚体,是不是应该出现很近的两条带才是??是不是应该比较靠下才对呢?那会不会出现没活化的32KD的呢?还有预染的MARKER 在15%的胶里大概会跑出多少KD 到多少KD 的几条带呢??我的TTBS忘记调PH了,今天

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