pLV3-U6-Scarf1-sgRNA1-Cas9-EGF

pLV3-U6-Scarf1-sgRNA1-Cas9-EGFP-BSD plasmid

PVT112032

pLV3-U6-Scarf1-sgRNA1-Cas9-EGFP-BSD plasmid Information

Plasmid name: pLV3-U6-Scarf1(mouse)-sgRNA1-Cas9-EGFP-BSD
Alias: TGGCATGGGCGACGACACGT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene knockout
Fragment type: CRISPR
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLV3-U6-Scarf1-sgRNA1-Cas9-EGFP-BSD plasmid Description

pLV3-U6-Scarf1-sgRNA1-Cas9-EGFP-BSD This plasmid is suitable for gene knockout in mammalian cells and can also be used for lentivirus packaging.

pLV3-U6-Scarf1-sgRNA1-Cas9-EGFP-BSD plasmid Sequence

1 GAGGGCCTAT TTCCCATGAT TCCTTCATAT TTGCATATAC GATACAAGGC TGTTAGAGAG 61 ATAATTAGAA TTAATTTGAC TGTAAACACA AAGATATTAG TACAAAATAC GTGACGTAGA 121 AAGTAATAAT TTCTTGGGTA GTTTGCAGTT TTAAAATTAT GTTTTAAAAT GGACTATCAT 181 ATGCTTACCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 241 CGAAACACCG TGGCATGGGC GACGACACGT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT 301 AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT CGGTGCTTTT TTGAATTCGC 361 TAGCTAGGTC TTGAAAGGAG TGGGAATTGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC 421 ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGATCCG GTGCCTAGAG 481 AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA 541 GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG 601 GTTTGCCGCC AGAACACAGG ACCGGTTCTA GAGCGCTGCC ACCATGGACA AGAAGTACAG 661 CATCGGCCTG GACATCGGCA CCAACTCTGT GGGCTGGGCC GTGATCACCG ACGAGTACAA 721 GGTGCCCAGC AAGAAATTCA AGGTGCTGGG CAACACCGAC CGGCACAGCA TCAAGAAGAA 781 CCTGATCGGA GCCCTGCTGT TCGACAGCGG CGAAACAGCC GAGGCCACCC GGCTGAAGAG 841 AACCGCCAGA AGAAGATACA CCAGACGGAA GAACCGGATC TGCTATCTGC AAGAGATCTT 901 CAGCAACGAG ATGGCCAAGG TGGACGACAG CTTCTTCCAC AGACTGGAAG AGTCCTTCCT 961 GGTGGAAGAG GATAAGAAGC ACGAGCGGCA CCCCATCTTC GGCAACATCG TGGACGAGGT 1021 GGCCTACCAC GAGAAGTACC CCACCATCTA CCACCTGAGA AAGAAACTGG TGGACAGCAC 1081 CGACAAGGCC GACCTGCGGC TGATCTATCT GGCCCTGGCC CACATGATCA AGTTCCGGGG 1141 CCACTTCCTG ATCGAGGGCG ACCTGAACCC CGACAACAGC GACGTGGACA AGCTGTTCAT 1201 CCAGCTGGTG CAGACCTACA ACCAGCTGTT CGAGGAAAAC CCCATCAACG CCAGCGGCGT 1261 GGACGCCAAG GCCATCCTGT CTGCCAGACT GAGCAAGAGC AGACGGCTGG AAAATCTGAT 1321 CGCCCAGCTG CCCGGCGAGA AGAAGAATGG CCTGTTCGGA AACCTGATTG CCCTGAGCCT 1381 GGGCCTGACC CCCAACTTCA AGAGCAACTT CGACCTGGCC GAGGATGCCA AACTGCAGCT 1441 GAGCAAGGAC ACCTACGACG ACGACCTGGA CAACCTGCTG GCCCAGATCG GCGACCAGTA 1501 CGCCGACCTG TTTCTGGCCG CCAAGAACCT GTCCGACGCC ATCCTGCTGA GCGACATCCT 1561 GAGAGTGAAC ACCGAGATCA CCAAGGCCCC CCTGAGCGCC TCTATGATCA AGAGATACGA 1621 CGAGCACCAC CAGGACCTGA CCCTGCTGAA AGCTCTCGTG CGGCAGCAGC TGCCTGAGAA 1681 GTACAAAGAG ATTTTCTTCG ACCAGAGCAA GAACGGCTAC GCCGGCTACA TTGACGGCGG 1741 AGCCAGCCAG GAAGAGTTCT ACAAGTTCAT CAAGCCCATC CTGGAAAAGA TGGACGGCAC 1801 CGAGGAACTG CTCGTGAAGC TGAACAGAGA GGACCTGCTG CGGAAGCAGC GGACCTTCGA 1861 CAACGGCAGC ATCCCCCACC AGATCCACCT GGGAGAGCTG CACGCCATTC TGCGGCGGCA 1921 GGAAGATTTT TACCCATTCC TGAAGGACAA CCGGGAAAAG ATCGAGAAGA TCCTGACCTT 1981 CCGCATCCCC TACTACGTGG GCCCTCTGGC CAGGGGAAAC AGCAGATTCG CCTGGATGAC 2041 CAGAAAGAGC GAGGAAACCA TCACCCCCTG GAACTTCGAG GAAGTGGTGG ACAAGGGCGC 2101 TTCCGCCCAG AGCTTCATCG AGCGGATGAC CAACTTCGAT AAGAACCTGC CCAACGAGAA 2161 GGTGCTGCCC AAGCACAGCC TGCTGTACGA GTACTTCACC GTGTATAACG AGCTGACCAA 2221 AGTGAAATAC GTGACCGAGG GAATGAGAAA GCCCGCCTTC CTGAGCGGCG AGCAGAAAAA 2281 GGCCATCGTG GACCTGCTGT TCAAGACCAA CCGGAAAGTG ACCGTGAAGC AGCTGAAAGA 2341 GGACTACTTC AAGAAAATCG AGTGCTTCGA CTCCGTGGAA ATCTCCGGCG TGGAAGATCG 2401 GTTCAACGCC TCCCTGGGCA CATACCACGA TCTGCTGAAA ATTATCAAGG ACAAGGACTT 2461 CCTGGACAAT GAGGAAAACG AGGACATTCT GGAAGATATC GTGCTGACCC TGACACTGTT 2521 TGAGGACAGA GAGATGATCG AGGAACGGCT GAAAACCTAT GCCCACCTGT TCGACGACAA 2581 AGTGATGAAG CAGCTGAAGC GGCGGAGATA CACCGGCTGG GGCAGGCTGA GCCGGAAGCT 2641 GATCAACGGC ATCCGGGACA AGCAGTCCGG CAAGACAATC CTGGATTTCC TGAAGTCCGA 2701 CGGCTTCGCC AACAGAAACT TCATGCAGCT GATCCACGAC GACAGCCTGA CCTTTAAAGA 2761 GGACATCCAG AAAGCCCAGG TGTCCGGCCA GGGCGATAGC CTGCACGAGC ACATTGCCAA 2821 TCTGGCCGGC AGCCCCGCCA TTAAGAAGGG CATCCTGCAG ACAGTGAAGG TGGTGGACGA 2881 GCTCGTGAAA GTGATGGGCC GGCACAAGCC CGAGAACATC GTGATCGAAA TGGCCAGAGA 2941 GAACCAGACC ACCCAGAAGG GACAGAAGAA CAGCCGCGAG AGAATGAAGC GGATCGAAGA 3001 GGGCATCAAA GAGCTGGGCA GCCAGATCCT GAAAGAACAC CCCGTGGAAA ACACCCAGCT 3061 GCAGAACGAG AAGCTGTACC TGTACTACCT GCAGAATGGG CGGGATATGT ACGTGGACCA 3121 GGAACTGGAC ATCAACCGGC TGTCCGACTA CGATGTGGAC CATATCGTGC CTCAGAGCTT 3181 TCTGAAGGAC GACTCCATCG ACAACAAGGT GCTGACCAGA AGCGACAAGA ACCGGGGCAA 3241 GAGCGACAAC GTGCCCTCCG AAGAGGTCGT GAAGAAGATG AAGAACTACT GGCGGCAGCT 3301 GCTGAACGCC AAGCTGATTA CCCAGAGAAA GTTCGACAAT CTGACCAAGG CCGAGAGAGG 3361 CGGCCTGAGC GAACTGGATA AGGCCGGCTT CATCAAGAGA CAGCTGGTGG AAACCCGGCA 3421 GATCACAAAG CACGTGGCAC AGATCCTGGA CTCCCGGATG AACACTAAGT ACGACGAGAA 3481 TGACAAGCTG ATCCGGGAAG TGAAAGTGAT CACCCTGAAG TCCAAGCTGG TGTCCGATTT 3541 CCGGAAGGAT TTCCAGTTTT ACAAAGTGCG CGAGATCAAC AACTACCACC ACGCCCACGA 3601 CGCCTACCTG AACGCCGTCG TGGGAACCGC CCTGATCAAA AAGTACCCTA AGCTGGAAAG 3661 CGAGTTCGTG TACGGCGACT ACAAGGTGTA CGACGTGCGG AAGATGATCG CCAAGAGCGA 3721 GCAGGAAATC GGCAAGGCTA CCGCCAAGTA CTTCTTCTAC AGCAACATCA TGAACTTTTT 3781 CAAGACCGAG ATTACCCTGG CCAACGGCGA GATCCGGAAG CGGCCTCTGA TCGAGACAAA 3841 CGGCGAAACC GGGGAGATCG TGTGGGATAA GGGCCGGGAT TTTGCCACCG TGCGGAAAGT 3901 GCTGAGCATG CCCCAAGTGA ATATCGTGAA AAAGACCGAG GTGCAGACAG GCGGCTTCAG 3961 CAAAGAGTCT ATCCTGCCCA AGAGGAACAG CGATAAGCTG ATCGCCAGAA AGAAGGACTG 4021 GGACCCTAAG AAGTACGGCG GCTTCGACAG CCCCACCGTG GCCTATTCTG TGCTGGTGGT 4081 GGCCAAAGTG GAAAAGGGCA AGTCCAAGAA ACTGAAGAGT GTGAAAGAGC TGCTGGGGAT 4141 CACCATCATG GAAAGAAGCA GCTTCGAGAA GAATCCCATC GACTTTCTGG AAGCCAAGGG 4201 CTACAAAGAA GTGAAAAAGG ACCTGATCAT CAAGCTGCCT AAGTACTCCC TGTTCGAGCT 4261 GGAAAACGGC CGGAAGAGAA TGCTGGCCTC TGCCGGCGAA CTGCAGAAGG GAAACGAACT 4321 GGCCCTGCCC TCCAAATATG TGAACTTCCT GTACCTGGCC AGCCACTATG AGAAGCTGAA 4381 GGGCTCCCCC GAGGATAATG AGCAGAAACA GCTGTTTGTG GAACAGCACA AGCACTACCT 4441 GGACGAGATC ATCGAGCAGA TCAGCGAGTT CTCCAAGAGA GTGATCCTGG CCGACGCTAA 4501 TCTGGACAAA GTGCTGTCCG CCTACAACAA GCACCGGGAT AAGCCCATCA GAGAGCAGGC 4561 CGAGAATATC ATCCACCTGT TTACCCTGAC CAATCTGGGA GCCCCTGCCG CCTTCAAGTA 4621 CTTTGACACC ACCATCGACC GGAAGAGGTA CACCAGCACC AAAGAGGTGC TGGACGCCAC 4681 CCTGATCCAC CAGAGCATCA CCGGCCTGTA CGAGACACGG ATCGACCTGT CTCAGCTGGG 4741 AGGCGACAAG CGACCTGCCG CCACAAAGAA GGCTGGACAG GCTAAGAAGA AGAAAGATTA 4801 CAAAGACGAT GACGATAAGG GATCCGGCGC AACAAACTTC TCTCTGCTGA AACAAGCCGG 4861 AGATGTCGAA GAGAATCCTG GACCGATGGT GTCTAAGGGC GAAGAGCTGT TCACCGGGGT 4921 GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG 4981 CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG 5041 CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT 5101 CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG 5161 CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA 5221 GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA 5281 GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA 5341 TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT 5401 CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG 5461 CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC 5521 CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT 5581 CGGCATGGAC GAGCTGTACA AGgagggcag aggaagtctt ctaacatgcg gtgacgtgga 5641 ggagaatccc ggccctatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag 5701 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc 5761 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc 5821 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg 5881 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca 5941 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc 6001 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctgactcga 6061 gTACGCGTTA AGTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA 6121 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC 6181 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT 6241 CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG 6301 CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT 6361 TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT 6421 GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT 6481 CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT 6541 ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC 6601 GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT 6661 CCCCGCGTCG ACTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT 6721 AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATCTGCTTTT 6781 TGCTTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT 6841 AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC 6901 CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA 6961 AATCTCTAGC AGGGCCCGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC 7021 AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA 7081 CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA 7141 TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC 7201 ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA 7261 GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 7321 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 7381 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 7441 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 7501 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 7561 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 7621 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 7681 AACAAAAATT TAACGCGAAT TAATTCTGTG GAATCACGTG CTACGAGATT TCGATTCCAC 7741 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT 7801 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC 7861 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 7921 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC 7981 GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 8041 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 8101 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 8161 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 8221 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 8281 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 8341 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 8401 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 8461 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 8521 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 8581 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 8641 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 8701 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 8761 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 8821 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 8881 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 8941 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 9001 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 9061 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 9121 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 9181 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 9241 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 9301 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 9361 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 9421 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 9481 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 9541 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 9601 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 9661 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 9721 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 9781 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 9841 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 9901 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 9961 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 10021 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 10081 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 10141 CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA 10201 TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT 10261 GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA 10321 GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGGGTTA GGCGTTTTGC GCTGCTTCGC 10381 GATGTACGGG CCAGATATAC GCGTTGACAT TGATTATTGA CTAGTTATTA ATAGTAATCA 10441 ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA 10501 AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT 10561 GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG 10621 TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC 10681 GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT 10741 CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG 10801 CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC 10861 ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT 10921 AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 10981 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 11041 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 11101 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 11161 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA 11221 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG 11281 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT 11341 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG 11401 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG 11461 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA 11521 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT 11581 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT 11641 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGCGGCCGCT 11701 GATCTTCAGA CCTGGAGGAG GAGATATGAG GGACAATTGG AGAAGTGAAT TATATAAATA 11761 TAAAGTAGTA AAAATTGAAC CATTAGGAGT AGCACCCACC AAGGCAAAGA GAAGAGTGGT 11821 GCAGAGAGAA AAAAGAGCAG TGGGAATAGG AGCTTTGTTC CTTGGGTTCT TGGGAGCAGC 11881 AGGAAGCACT ATGGGCGCAG CGTCAATGAC GCTGACGGTA CAGGCCAGAC AATTATTGTC 11941 TGGTATAGTG CAGCAGCAGA ACAATTTGCT GAGGGCTATT GAGGCGCAAC AGCATCTGTT 12001 GCAACTCACA GTCTGGGGCA TCAAGCAGCT CCAGGCAAGA ATCCTGGCTG TGGAAAGATA 12061 CCTAAAGGAT CAACAGCTCC TGGGGATTTG GGGTTGCTCT GGAAAACTCA TTTGCACCAC 12121 TGCTGTGCCT TGGAATGCTA GTTGGAGTAA TAAATCTCTG GAACAGATTT GGAATCACAC 12181 GACCTGGATG GAGTGGGACA GAGAAATTAA CAATTACACA AGCTTAATAC ACTCCTTAAT 12241 TGAAGAATCG CAAAACCAGC AAGAAAAGAA TGAACAAGAA TTATTGGAAT TAGATAAATG 12301 GGCAAGTTTG TGGAATTGGT TTAACATAAC AAATTGGCTG TGGTATATAA AATTATTCAT 12361 AATGATAGTA GGAGGCTTGG TAGGTTTAAG AATAGTTTTT GCTGTACTTT CTATAGTGAA 12421 TAGAGTTAGG CAGGGATATT CACCATTATC GTTTCAGACC CACCTCCCAA CCCCGAGGGG 12481 ACCCGACAGG CCCGAAGGAA TAGAAGAAGA AGGTGGAGAG AGAGACAGAG ACAGATCCAT 12541 TCGATTAGTG AACGGATCGG CACTGCGTGC GCCAATTCTG CAGACAAATG GCAGTATTCA 12601 TCCACAATTT TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG 12661 ACATAATAGC AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA 12721 ATTTTCGGGT TTATTACAGG GACAGCAGAG ATCCAGTTTG GTTAATTAAG GTACC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.