pCMV-Gnl2-3×FLAG-Neo plasmid

pCMV-Gnl2-3×FLAG-Neo plasmid

PVT106026

pCMV-Gnl2-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Gnl2(mouse)-3×FLAG-Neo
Alias: NM_145552.2;HUMAUANTIG; Ngp-1;
Gene lenght: 2184
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Gnl2-3×FLAG-Neo plasmid Description

pCMV-Gnl2-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Gnl2-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatggt gaagcccaag tacaaaggac 781 ggagcaccat caaccgctcg gcggccagca ccaacccaga tcgagtacag ggagctggcg 841 gccaaaacat gagggatcgg ggcacaattc ggcgcctgaa tatgtacagg caaaaggagc 901 gcaggaacag tcgtggtaaa gtgattaagc cactgcagta tcagtcaact gtggcttctg 961 gcacagtggc ccgagtggag ccgaatatta aatggtttgg aaatactcgt gtgatcaagc 1021 aggcatcatt acaaaaattt caagaggaaa tggataaagt tatgaaggat ccatacaaag 1081 ttgtcatgaa acaaagcaaa ttaccgatgt ctcttctgca cgatcgaatc cagcctcata 1141 acgcaaaagt ccacattctt gatactgaaa gctttgaaag tacatttggc ccaaagtcac 1201 agagaaagcg gccaaacttg tttgcaagtg atatgcaatc ccttctagaa aacgctgaaa 1261 tgtctactga gagctatgac cagggcaagg accgtgattt ggtgatggag gacactggtg 1321 taagaaatga agctcaagaa gagatatata aaaaagggca gtcaaaaaga atatggggag 1381 aactctacaa ggtgatagac tcatcagatg ttgtcgttca agtccttgac gctagagatc 1441 cgatgggcac tcgttccccc cacatcgaag cttacttgaa aaaggaaaaa ccctggaaac 1501 atctcatttt tgtactcaat aagtgtgacc ttgttccaac ttgggcaacc aaacgatggg 1561 ttgctgtgct ctcccaggac tacccaacac tggctttcca tgcgagcctc accaatccct 1621 ttgggaaggg agcattcatt cagcttctgc ggcagtttgg gaagttgcac acagacaaga 1681 aacaaatcag tgttgggttc attggctatc caaatgtagg caagagctct gtgattaata 1741 cattacgatc caagaaagtt tgcaacgtgg cccccattgc tggagaaaca aaggtctggc 1801 agtatattac cttgatgcgt cgtatattcc tgattgactg ccctggtgtg gtttacccat 1861 ctgaggactc agagaccgac attgtgctca aaggagtggt tcaagttgag aaaattaaag 1921 ctcctcaaga ccacattggt gctgtccttg aacgagcaaa gccagagtat atcagcaaga 1981 cgtacaagat tgagtcctgg gagaacgcgg aggactttct tgagaagcta gctctccgca 2041 ctgggaagtt actgaagggt ggagagcctg acatgctgac tgtgagcaag atggttctca 2101 atgactggca gagaggccga atccctttct ttgtcaagcc gcccaatgca gagctaccga 2161 ccgattccca gcttccacca tcctcaccat tggaagttcc cacagaaaca acccagaaca 2221 acccagaaga agagaccaca gaaacagaag ttgaaaggtc agactctatc actgaaaagg 2281 agccagaagg agactgttct caggatagaa actcagagat gcaacagatc ctcgcacgag 2341 ttcgccagaa ctttggcaaa atcaacgtgg ggcctcagtt ttctgcggat gacctggtgc 2401 ctgtggagat gtcagacttg gaagatctgg aaagctctgg ggaagaggaa gaacaggagc 2461 aggaacagcc aggggaggat gccgaggaag agcgctcccc agacactcag gaggaaccag 2521 tgggaaacga caccaaggcc gtgctcagag ccctggatga gaagattgcc aagtaccaga 2581 ggtttctaaa taaagctaaa gctaaaaagt tctctgccgt cagaatatcc aaggacttaa 2641 gtgaaaaggt ttttgcaaaa tacaaagaag agaagaaaac atctgcagaa gacagtgatg 2701 cagcacccac caaaaaggca aggaagtggg atgcacagat ggaagaagaa ccttcaaata 2761 agactcagag gatgctgacg tgtaaggaac ggaggagagc agcacggcag caacaatcca 2821 aaaaagttgg tgtgcgttac tacgagacac acaatgtgaa aaacaggaac aggaacaaaa 2881 agaagacgag cgactcagag ggacagaaac acagacgcaa caagttcaga cagaagcagG 2941 GATCCGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG 3001 ACGATGACAA GTAGTGAGGG CCCGATATCT CGACAATCAA CCTCTGGATT ACAAAATTTG 3061 TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC 3121 TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA 3181 TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT 3241 GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA 3301 GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC 3361 CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT 3421 GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG 3481 CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG 3541 CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT 3601 CTCCCTTTGG GCCGCCTCCC CGCCTGGAAA CGGGGGAGGC TAACTGAAAC ACGGAAGGAG 3661 ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGG 3721 TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC 3781 CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC 3841 CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT 3901 AGCAGATCTG CGCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT 3961 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC 4021 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 4081 AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC 4141 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT 4201 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC 4261 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC 4321 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT 4381 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 4441 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 4501 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 4561 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 4621 TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 4681 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 4741 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 4801 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 4861 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 4921 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 4981 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 5041 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 5101 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 5161 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 5221 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 5281 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 5341 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 5401 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 5461 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 5521 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 5581 TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC 5641 CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT 5701 GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC 5761 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT 5821 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT 5881 ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA 5941 TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG 6001 GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA 6061 GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG 6121 TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG 6181 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 6241 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 6301 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 6361 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 6421 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 6481 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC 6541 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 6601 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 6661 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 6721 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC 6781 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 6841 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 6901 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 6961 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 7021 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 7081 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 7141 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 7201 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 7261 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 7321 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 7381 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 7441 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 7501 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 7561 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 7621 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 7681 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 7741 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 7801 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 7861 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 7921 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 7981 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 8041 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC 8101 CTATGGTCGA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC 8161 CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG 8221 CAAGGCTTGA CCGACAATTG CATGAAGAAT CTGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.