pAAV-CMV-Aqp4-T2A-ZsGreen1 pla

pAAV-CMV-Aqp4-T2A-ZsGreen1 plasmid

PVT111717

pAAV-CMV-Aqp4-T2A-ZsGreen1 plasmid Information

Plasmid name: pAAV-CMV-Aqp4(mouse)-T2A-ZsGreen1
Alias: NM_001317729.1;WCH4
Gene lenght: 1056
Host: Mammalian cells,Adeno associated virus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pAAV-CMV-Aqp4-T2A-ZsGreen1 plasmid Description

pAAV-CMV-Aqp4-T2A-ZsGreen1 is a plasmid suitable for gene expression in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-CMV-Aqp4-T2A-ZsGreen1 plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtGACAT TGATTATTGA CTAGTTATTA 181 ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA 241 ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT 301 AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA 361 GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC 421 CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT 481 ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT 541 GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG 601 TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC 661 AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA 721 GGTCTATATA AGCAGAGCTg gtaccGCCAC Catgagtgac agagctgcgg caaggcggtg 781 gggtaagtgt ggacattcct gcagtagaga gagcatcatg gtggctttca aaggagtctg 841 gactcaggct ttctggaagg cagtctcagc agaatttctg gccacgctta tctttgtttt 901 gctcggtgtg ggatccacca taaactgggg tggctcagaa aaccccttac ctgtggacat 961 ggtcctcatc tccctttgct ttggactcag cattgctacc atggtgcagt gctttggcca 1021 catcagtggt ggccacatca atcccgctgt gactgtagcc atggtgtgca cacgaaagat 1081 cagcatcgct aagtccgtct tctacatcat tgcacagtgc ctgggggcca tcattggagc 1141 cggcatcctc tacctggtca cacctcccag tgtggttgga ggattgggag tcaccacggt 1201 tcatggaaac ctcaccgctg gccatgggct cctggtggag ttaataatca ctttccagtt 1261 ggtgttcact atttttgcca gctgtgattc caaacgaact gatgttactg gttcaatagc 1321 tttagcaatt ggattttccg ttgcaattgg acatttgttt gcaatcaatt atactggagc 1381 cagcatgaat ccagctcgat cttttggacc cgcagttatc atgggaaact gggcaaacca 1441 ctggatatat tgggttggac caatcatggg cgctgtgctg gcaggtgccc tttatgagta 1501 tgtcttctgt cctgatgtgg agctcaaacg tcgccttaag gaagccttca gcaaagccgc 1561 gcagcagaca aaagggagct acatggaggt ggaggacaac cggagccaag tggagacgga 1621 agacttgatc ctgaagcccg gagtggtgca tgtgattgac attgaccgtg gagaagagaa 1681 gaaggggaaa gactcttcgg gagaggtatt gtcttccgta tgactagagg acagcactga 1741 aggcagaaga gactccctag acctggcctc agatttcctg ccacccatta aggaaacaga 1801 tttgttaggt ctagagtcga cagagggcag aggaagtctt ctaacatgcg gtgacgtgga 1861 ggagaatccc ggcccttccg gaaccatggc ccagtccaag cacggcctga ccaaggagat 1921 gaccatgaag taccgcatgg agggctgcgt ggacggccac aagttcgtga tcaccggcga 1981 gggcatcggc taccccttca agggcaagca ggccatcaac ctgtgcgtgg tggagggcgg 2041 ccccttgccc ttcgccgagg acatcttgtc cgccgccttc atgtacggca accgcgtgtt 2101 caccgagtac ccccaggaca tcgtcgacta cttcaagaac tcctgccccg ccggctacac 2161 ctgggaccgc tccttcctgt tcgaggacgg cgccgtgtgc atctgcaacg ccgacatcac 2221 cgtgagcgtg gaggagaact gcatgtacca cgagtccaag ttctacggcg tgaacttccc 2281 cgccgacggc cccgtgatga agaagatgac cgacaactgg gagccctcct gcgagaagat 2341 catccccgtg cccaagcagg gcatcttgaa gggcgacgtg agcatgtacc tgctgctgaa 2401 ggacggtggc cgcttgcgct gccagttcga caccgtgtac aaggccaagt ccgtgccccg 2461 caagatgccc gactggcact tcatccagca caagctgacc cgcgaggacc gcagcgacgc 2521 caagaaccag aagtggcacc tgaccgagca cgccatcgcc tccggctccg ccttgccctg 2581 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 2641 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 2701 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 2761 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 2821 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 2881 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 2941 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 3001 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 3061 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 3121 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 3181 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga 3241 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt 3301 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg 3361 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg 3421 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc 3481 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg 3541 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc 3601 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct 3661 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac 3721 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc 3781 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg 3841 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt 3901 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc 3961 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 4021 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 4081 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 4141 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc 4201 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 4261 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga 4321 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 4381 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg 4441 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 4501 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 4561 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 4621 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 4681 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 4741 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 4801 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 4861 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 4921 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 4981 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 5041 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 5101 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 5161 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 5221 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 5281 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 5341 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 5401 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 5461 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 5521 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 5581 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 5641 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 5701 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 5761 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 5821 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 5881 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 5941 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 6001 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6061 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 6121 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 6181 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 6241 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 6301 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 6361 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 6421 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.