pLV3-CMV-CDX1-3×FLAG-CopGFP-Pu

pLV3-CMV-CDX1-3×FLAG-CopGFP-Puro plasmid

PVT96867

pLV3-CMV-CDX1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CDX1(human)-3×FLAG-CopGFP-Puro
Alias: NM_001804.3;
Gene lenght: 795bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CDX1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-CDX1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CDX1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt atgtgggcta tgtgctggac 2341 aaggattcgc ccgtgtaccc cggcccagcc aggccagcca gcctcggcct gggcccgcaa 2401 gcctacggcc ccccggcccc gcccccggcg cccccgcagt accccgactt ctccagctac 2461 tctcacgtgg agccggcccc cgcgcccccg acggcctggg gggcgccctt ccctgcgccc 2521 aaggacgact gggccgccgc ctacggcccg ggccccgcgg cccctgccgc cagcccagct 2581 tcgctggcat tcgggccccc tccagacttt agcccggtgc cggcgccccc tgggcccggc 2641 ccgggcctcc tggcgcagcc cctcgggggc ccgggcacac cgtcctcgcc cggagcgcag 2701 aggccgacgc cctacgagtg gatgcggcgc agcgtggcgg ccggaggcgg cggtggcagc 2761 ggtaagactc ggaccaagga caagtaccgc gtggtctaca ccgaccacca acgcctggag 2821 ctggagaagg agtttcatta cagccgttac atcacaatcc ggcggaaatc agagctggct 2881 gccaatctgg ggctcactga acggcaggtg aagatctggt tccaaaaccg gcgggcaaag 2941 gagcgcaaag tgaacaagaa gaaacagcag cagcaacagc ccccacagcc gccgatggcc 3001 cacgacatca cggccacccc agccgggcca tccctggggg gcctgtgtcc cagcaacacc 3061 agcctcctgg ccacctcctc tccaatgcct gtgaaagagg agtttctgcc aggatccGAC 3121 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 3181 AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 3241 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 3301 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 3361 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 3421 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 3481 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 3541 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 3601 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 3661 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 3721 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc 3781 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg 3841 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa 3901 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac 3961 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc 4021 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc 4081 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc 4141 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg 4201 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac 4261 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc 4321 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc 4381 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc 4441 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc 4501 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 4561 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 4621 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 4681 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 4741 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 4801 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 4861 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 4921 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 4981 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 5041 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 5101 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 5161 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 5221 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 5281 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 5341 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 5401 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 5461 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 5521 ctgttgggca ctgacaattc cgtggtgttg tcggggaaAT CATCGTCCTT TCCTtggctg 5581 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 5641 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 5701 ctTcgccttc gccctcagac gagtcggatc tccctttggG ccgcctcccc gcctggtacc 5761 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 5821 gggactggaa gggctaattc actcccaacg aaGataagat ctgctttttg cttgtactgg 5881 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 5941 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 6001 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 6061 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 6121 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 6181 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 6241 caatgtatct tatcatgtct ggctctagct aTCCCGCCCC TAACTCCGCC CAtcccgccc 6301 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 6361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 6421 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg 6481 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 6541 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 6601 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 6661 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6721 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6781 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 6841 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 6901 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 6961 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 7021 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 7081 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7141 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7201 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7261 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7321 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7381 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7441 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 7501 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 7561 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 7621 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 7681 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 7741 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 7801 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 7861 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 7921 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 7981 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 8041 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 8101 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 8161 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 8221 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 8281 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 8341 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 8401 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 8461 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 8521 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 8581 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 8641 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 8701 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 8761 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 8821 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 8881 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 8941 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 9001 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 9061 taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.