pLV3-CMV-BHLHE41-3×HA-EF1a-Bla

pLV3-CMV-BHLHE41-3×HA-EF1a-Blast plasmid

PVT95446

pLV3-CMV-BHLHE41-3×HA-EF1a-Blast plasmid Information

Plasmid name: pLV3-CMV-BHLHE41(human-gc-opt)-3×HA-EF1a-Blast
Alias: NM_030762.3;BHLHB3; DEC2; FNSS1; hDEC2; SHARP1
Gene lenght: 1446bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-BHLHE41-3×HA-EF1a-Blast plasmid Description

pLV3-CMV-BHLHE41-3×HA-EF1a-Blast is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-BHLHE41-3×HA-EF1a-Blast plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggacgaa ggaattcctc atttgcaaga gagacagtta 2341 ctggaacata gagattttat aggactggac tattcctctt tgtatatgtg taaacccaaa 2401 aggagcatga aacgagacga caccaaggat acctacaaat taccgcacag attaatagaa 2461 aagaaaagaa gagaccgaat taatgaatgc attgctcagc tgaaagattt actgcctgaa 2521 catctgaaat tgacaactct gggacatctg gagaaagctg tagtcttgga attaactttg 2581 aaacacttaa aagctttaac cgccttaacc gagcaacagc atcagaagat aattgcttta 2641 cagaatgggg agcgatctct gaaatcgccc attcagtccg acttggatgc gttccactcg 2701 ggatttcaaa catgcgccaa agaagtcttg caatacctct cccggtttga gagctggaca 2761 cccagggagc cgcggtgtgt ccagctgatc aaccacttgc acgccgtggc cacccagttc 2821 ttgcccaccc cgcagctgtt gactcaacag gtccctctga gcaaaggcac cggcgctccc 2881 tcggccgccg ggtccgcggc cgccccctgc ctggagcgcg cggggcagaa gctggagccc 2941 ctcgcctact gcgtgcccgt catccagcgg actcagccca gcgccgagct cgccgccgag 3001 aacgacacgg acaccgacag cggctacggc ggcgaagccg aggcccggcc ggaccgcgag 3061 aaaggcaaag gcgcgggggc gagccgcgtc accatcaagc aggagcctcc cggggaggac 3121 tcgccggcgc ccaagaggat gAAGCTGGAC AGCAGAGGCG GCGGAAGCGG CGGAGGTCCA 3181 GGCGGTGGAG CTGCTGCCGC TGCTGCTGCT CTGCTGGGAC CTGACCCTGC TGCTGCTGCC 3241 GCTCTGCTTA GACCTGACGC TGCCTTGCTC AGCTCTCTGG TGGCCTTCGG CGGAGGCGGA 3301 GGTGCTCCAT TTCCACAGCC TGCTGCTGCT GCCGCTCCAT TCTGCCTGCC TTTCTGCTTT 3361 CTGTCTCCAA GCGCTGCCGC TGCTTACGTC CAGCCTTTCT TGGACAAGAG CGGCCTGGAG 3421 AAGTACCTGT ATCCAGCCGC TGCTGCCGCT CCATTCCCTC TGCTGTATCC AGGCATACCT 3481 GCTCCAGCTG CTGCTGCCGC TGCTGCTGCT GCTGCTGCTG CTGCTGCCGC TGCCTTTCCA 3541 TGCCTGAGCA GCGTGCTGTC TCCACCACCT GAGAAGGCTG GAGCTGCTGC TGCCACACTG 3601 TTGCCTCACG AAGTGGCTCC ACTGGGAGCA CCTCATCCAC AGCATCCACA CGGCAGAACA 3661 CACCTGCCTT TCGCTGGACC TAGAGAGCCT GGCAATCCAG AGTCTAGCGC TCAAGAAGAT 3721 CCATCTCAGC CTGGCAAAGA GGCACCTCTC GAGTACCCAT ACGACGTACC TGATTATGCA 3781 ACTTATCCTT ATGATGTACC TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA 3841 TAGGGATCCG CGGCCGCGAA GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG 3901 CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA 3961 GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC 4021 CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA 4081 CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC 4141 CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC 4201 CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC 4261 GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG 4321 CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG 4381 ATCCAAGCTG TGACCGGCGC CTACGCTAGG CCACCATGGC CAAGCCTTTG TCTCAAGAAG 4441 AATCCACCCT CATTGAAAGA GCAACGGCTA CAATCAACAG CATCCCCATC TCTGAAGACT 4501 ACAGCGTCGC CAGCGCAGCT CTCTCTAGCG ACGGCCGCAT CTTCACTGGT GTCAATGTAT 4561 ATCATTTTAC TGGGGGACCT TGTGCAGAAC TCGTGGTGCT GGGCACTGCT GCTGCTGCGG 4621 CAGCTGGCAA CCTGACTTGT ATCGTCGCGA TCGGAAATGA GAACAGGGGC ATCTTGAGCC 4681 CCTGCGGACG GTGCCGACAG GTGCTTCTCG ATCTGCATCC TGGGATCAAA GCCATAGTGA 4741 AGGACAGTGA TGGACAGCCG ACGGCAGTTG GGATTCGTGA ATTGCTGCCC TCTGGTTATG 4801 TGTGGGAGGG CTAAGTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 4861 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 4921 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 4981 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 5041 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 5101 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 5161 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT 5221 CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 5281 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 5341 TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG 5401 CCTCCCCGCC TGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC 5461 TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAA ATAAGATCTG 5521 CTTTTTGCTT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC 5581 TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG 5641 TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG 5701 TGGAAAATCT CTAGCAGTAG TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA 5761 AAGAAATGAA TATCAGAGAG TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA 5821 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT 5881 GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA 5941 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG 6001 AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG 6061 GCCTAGACTT TTGCAGAGAC GGCCCAAATT CGTAATCATG GTCATAGCTG TTTCCTGTGT 6121 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 6181 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 6241 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 6301 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 6361 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 6421 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 6481 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 6541 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 6601 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 6661 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 6721 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 6781 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 6841 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 6901 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT 6961 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 7021 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 7081 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 7141 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 7201 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 7261 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 7321 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 7381 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 7441 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 7501 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 7561 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 7621 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 7681 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 7741 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 7801 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 7861 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 7921 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 7981 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 8041 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 8101 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 8161 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 8221 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA 8281 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG 8341 ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG 8401 ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT 8461 GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA 8521 TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC 8581 GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG 8641 GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT 8701 GTAAAACGAC GGCCAGTGCC AAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.