pLV3-CMV-PRKCB-3×FLAG-CopGFP-P

pLV3-CMV-PRKCB-3×FLAG-CopGFP-Puro plasmid

PVT94629

pLV3-CMV-PRKCB-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PRKCB(human)-3×FLAG-CopGFP-Puro
Alias: NM_212535.3;PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1
Gene lenght: 2013bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PRKCB-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-PRKCB-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-PRKCB-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ctgacccggc tgcggggccg 2341 ccgccgagcg agggcgagga gagcaccgtg cgcttcgccc gcaaaggcgc cctccggcag 2401 aagaacgtgc atgaggtcaa gaaccacaaa ttcaccgccc gcttcttcaa gcagcccacc 2461 ttctgcagcc actgcaccga cttcatctgg ggcttcggga agcagggatt ccagtgccaa 2521 gtttgctgct ttgtggtgca caagcggtgc catgaatttg tcacattctc ctgccctggc 2581 gctgacaagg gtccagcctc cgatgacccc cgcagcaaac acaagtttaa gatccacacg 2641 tactccagcc ccacgttttg tgaccactgt gggtcactgc tgtatggact catccaccag 2701 gggatgaaat gtgacacctg catgatgaat gtgcacaagc gctgcgtgat gaatgttccc 2761 agcctgtgtg gcacggacca cacggagcgc cgcggccgca tctacatcca ggcccacatc 2821 gacagggacg tcctcattgt cctcgtaaga gatgctaaaa accttgtacc tatggacccc 2881 aatggcctgt cagatcccta cgtaaaactg aaactgattc ccgatcccaa aagtgagagc 2941 aaacagaaga ccaaaaccat caaatgctcc ctcaaccctg agtggaatga gacatttaga 3001 tttcagctga aagaatcgga caaagacaga agactgtcag tagagatttg ggattgggat 3061 ttgaccagca ggaatgactt catgggatct ttgtcctttg ggatttctga acttcagaaa 3121 gccagtgttg atggctggtt taagttactg agccaggagg aaggcgagta cttcaatgtg 3181 cctgtgccac cagaaggaag tgaggccaat gaagaactgc ggcagaaatt tgagagggcc 3241 aagatcagtc agggaaccaa ggtcccggaa gaaaagacga ccaacactgt ctccaaattt 3301 gacaacaatg gcaacagaga ccggatgaaa ctgaccgatt ttaacttcct aatggtgctg 3361 gggaaaggca gctttggcaa ggtcatgctt tcagaacgaa aaggcacaga tgagctctat 3421 gctgtgaaga tcctgaagaa ggacgttgtg atccaagatg atgacgtgga gtgcactatg 3481 gtggagaagc gggtgttggc cctgcctggg aagccgccct tcctgaccca gctccactcc 3541 tgcttccaga ccatggaccg cctgtacttt gtgatggagt acgtgaatgg gggcgacctc 3601 atgtatcaca tccagcaagt cggccggttc aaggagcccc atgctgtatt ttacgctgca 3661 gaaattgcca tcggtctgtt cttcttacag agtaagggca tcatttaccg tgacctaaaa 3721 cttgacaacg tgatgctcga ttctgaggga cacatcaaga ttgccgattt tggcatgtgt 3781 aaggaaaaca tctgggatgg ggtgacaacc aagacattct gtggcactcc agactacatc 3841 gcccccgaga taattgctta tcagccctat gggaagtccg tggattggtg ggcatttgga 3901 gtcctgctgt atgaaatgtt ggctgggcag gcaccctttg aaggggagga tgaagatgaa 3961 ctcttccaat ccatcatgga acacaacgta gcctatccca agtctatgtc caaggaagct 4021 gtggccatct gcaaagggct gatgaccaaa cacccaggca aacgtctggg ttgtggacct 4081 gaaggcgaac gtgatatcaa agagcatgca tttttccggt atattgattg ggagaaactt 4141 gaacgcaaag agatccagcc cccttataag ccaaaagcta gagacaagag agacacctcc 4201 aacttcgaca aagagttcac cagacagcct gtggaactga cccccactga taaactcttc 4261 atcatgaact tggaccaaaa tgaatttgct ggcttctctt atactaaccc agagtttgtc 4321 attaatgtgg gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC 4381 TACAAGGATG ACGATGACAA Gtagcggccg cGaaggatct gcgatcgctc cggtgcccgt 4441 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 4501 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 4561 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 4621 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc 4681 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt 4741 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag 4801 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg 4861 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt 4921 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc 4981 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc 5041 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac 5101 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg 5161 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac 5221 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg 5281 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg 5341 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac 5401 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc 5461 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc 5521 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc 5581 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag 5641 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc 5701 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac 5761 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc 5821 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 5881 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 5941 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 6001 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 6061 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 6121 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 6181 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 6241 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 6301 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 6361 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact 6421 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 6481 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 6541 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 6601 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 6661 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 6721 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaATCA 6781 TCGTCCTTTC CTtggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 6841 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 6901 ctgcggcctc ttccgcgtct Tcgccttcgc cctcagacga gtcggatctc cctttggGcc 6961 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 7021 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa Gataagatct 7081 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 7141 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 7201 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 7261 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 7321 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 7381 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 7441 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctaT CCCGCCCCTA 7501 ACTCCGCCCA tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 7561 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 7621 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc 7681 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 7741 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 7801 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 7861 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 7921 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 7981 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 8041 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 8101 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 8161 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 8221 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 8281 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 8341 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 8401 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 8461 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 8521 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 8581 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 8641 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 8701 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 8761 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 8821 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 8881 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 8941 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 9001 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 9061 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 9121 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 9181 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 9241 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 9301 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 9361 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 9421 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 9481 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 9541 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 9601 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 9661 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 9721 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 9781 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 9841 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 9901 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 9961 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 10021 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 10081 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca 10141 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 10201 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 10261 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.