pLV3-CMV-Ccl2-3×FLAG-CopGFP-Pu

pLV3-CMV-Ccl2-3×FLAG-CopGFP-Puro plasmid

PVT86876

pLV3-CMV-Ccl2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Ccl2(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_011333.3;HC11; JE; MCAF; MCP-1; MCP1; Scya2; Sigje;SMC-CF;
Gene lenght: 444bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Ccl2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Ccl2-3×FLAG-CopGFP-Puro This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate transcription of the mouse Ccl2 gene, promoting the C-terminal fusion expression of a 3×FLAG tag, which facilitates detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, allowing for stable integration of the gene into host cells. The plasmid carries the CopGFP green fluorescent protein for observing the expression and localization of the target gene. The plasmid contains a Puro resistance gene for selecting host cells that express the target gene.

pLV3-CMV-Ccl2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc aggtccctgt catgcttctg 2341 ggcctgctgt tcacagttgc cggctggagc atccacgtgt tggctcagcc agatgcagtt 2401 aacgccccac tcacctgctg ctactcattc accagcaaga tgatcccaat gagtaggctg 2461 gagagctaca agaggatcac cagcagcagg tgtcccaaag aagctgtagt ttttgtcacc 2521 aagctcaaga gagaggtctg tgctgacccc aagaaggaat gggtccagac atacattaaa 2581 aacctggatc ggaaccaaat gagatcagaa cctacaactt tatttaaaac tgcatctgcc 2641 ctaaggtctt cagcaccttt gaatgtgaag ttgacccgta aatctgaagc taatgcatcc 2701 actacctttt ccacaaccac ctcaagcact tctgtaggag tgaccagtgt gacagtgaac 2761 ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 2821 GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 2881 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 2941 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 3001 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 3061 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 3121 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 3181 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 3241 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 3301 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 3361 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 3421 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 3481 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 3541 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 3601 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 3661 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 3721 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 3781 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 3841 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 3901 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 3961 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 4021 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 4081 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 4141 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 4201 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 4261 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 4321 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 4381 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 4441 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 4501 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 4561 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 4621 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 4681 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 4741 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 4801 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 4861 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 4921 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 4981 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 5041 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 5101 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 5161 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC ATCGTCCTTT 5221 CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 5281 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 5341 cttccgcgtc tTcgccttcg ccctcagacg agtcggatct ccctttggGc cgcctccccg 5401 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 5461 agaaaagggg ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc 5521 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 5581 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 5641 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 5701 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 5761 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 5821 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 5881 caaactcatc aatgtatctt atcatgtctg gctctagcta TCCCGCCCCT AACTCCGCCC 5941 Atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 6001 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 6061 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt 6121 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 6181 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 6241 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 6301 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 6361 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 6421 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 6481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 6541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 6601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 6661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 6721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 6781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 6841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 6901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 6961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7141 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 7201 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 7261 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 7321 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 7381 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 7441 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 7501 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 7561 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 7621 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 7681 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 7741 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 7801 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 7861 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 7921 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 7981 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 8041 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 8101 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 8161 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 8221 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 8281 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 8341 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 8401 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 8461 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 8521 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 8581 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 8641 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 8701 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.