pMSCV-Tfeb-3×FLAG-PGK-Puro pla

pMSCV-Tfeb-3×FLAG-PGK-Puro plasmid

PVT96574

pMSCV-Tfeb-3×FLAG-PGK-Puro plasmid Information

Plasmid name: pMSCV-Tfeb(mouse)-3×FLAG-PGK-Puro
Alias: NM_011549.4;bHLHe35; Tcfeb
Gene lenght: 1425bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: Non
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: PLXSN-F
Reverse primer:
Copy number: High
Induction:

pMSCV-Tfeb-3×FLAG-PGK-Puro plasmid Description

pMSCV-Tfeb-3×FLAG-PGK-Puro This is a plasmid suitable for protein expression in mammalian cells and can also be used for retrovirus packaging.

pMSCV-Tfeb-3×FLAG-PGK-Puro plasmid Sequence

1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT 61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA 121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA 181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT 241 CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC 301 GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC 361 CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA 421 AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA 481 GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA 541 CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG 601 TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT 661 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA 721 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA 781 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG 841 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA 901 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG 961 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT 1021 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA 1081 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG 1141 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC 1201 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC 1261 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG 1321 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG 1381 CCCTCACTCC TTCTCTAGGC GCCGGAATTA GATCTCTCGA GGCCACCatg gcgtcacgca 1441 tcgggctgcg catgcagctc atgcgggagc aggcccagca ggaggagcag cgagagcgca 1501 tgcagcagca ggctgtcatg cattatatgc aacagcagca gcagcagcag cagcagctgg 1561 gtgggccccc caccccagcc atcaacaccc ctgtccactt ccagtcgccc ccgcctgtgc 1621 ccggggaggt gctgaaggtg cagtcctacc tggagaaccc cacctcctac cacctgcaac 1681 agtcccagca tcagaaggtt cgggagtatc tgtctgagac ctatgggaac aagtttgctg 1741 cccacgtgag cccagcccaa ggttccccga agcctgcccc agcagcatcc ccaggggtgc 1801 gggctggaca cgtactgtcc acctcggccg gcaacagtgc tcccaacagt cccatggcca 1861 tgctacatat cagctccaac cccgagaaag agtttgatga tgtcattgac aacattatgc 1921 gcctggacag cgtgctgggc tacatcaacc ctgagatgca gatgcctaac acgctgcccc 1981 tgtctagcag ccacctgaac gtgtacagcg gtgaccccca ggtcacagcc tccatggtgg 2041 gtgtcaccag cagctcctgc cctgccgacc tgactcagaa gcgagagcta acagatgctg 2101 agagcagagc cctggccaag gagcggcaga agaaagacaa tcacaaccta attgagagaa 2161 gacgcaggtt caacatcaat gaccggatca aggagctggg aatgctgatc cccaaggcca 2221 acgacctgga cgtgcgctgg aacaaaggca ccatcctcaa ggcctctgtg gattacatcc 2281 ggaggatgca gaaggacctg cagaagtccc gggagctgga gaaccactcc cggcgcctgg 2341 agatgactaa caagcagctc tggctccgca tccaggagct ggagatgcag gcacgcgtgc 2401 acggcctccc caccacctcg ccgtcgggtg tgaatatggc cgagctggcc cagcaggtgg 2461 tgaagcaaga gttgcccagt gaggatggcc caggggaggc gctgatgctg gggcctgagg 2521 tccctgagcc tgagcaaatg ccggctcttc ctccccaggc tccgctgccc tcggccgccc 2581 agccacagtc tccgttccat cacctggact tcagccatgg cctgagcttt gggggtgggg 2641 gcgacgaggg gcccacaggt taccccgata ccctggggac agagcacggc tccccattcc 2701 ccaacctgtc caagaaggat ctggacttaa tgctcctaga tgactccctg ctccccctgg 2761 cctctgaccc cctcttttct accatgtctc ctgaggcctc caaggccagc agccgccgga 2821 gcagcttcag catggaggag ggtgatgttc tgGACTACAA AGACCATGAC GGTGATTATA 2881 AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA GGAATTCTAC CGGGTAGGGG 2941 AGGCGCTTTT CCCAAGGCAG TCTGGAGCAT GCGCTTTAGC AGCCCCGCTG GGCACTTGGC 3001 GCTACACAAG TGGCCTCTGG CCTCGCACAC ATTCCACATC CACCGGTAGG CGCCAACCGG 3061 CTCCGTTCTT TGGTGGCCCC TTCGCGCCAC CTTCTACTCC TCCCCTAGTC AGGAAGTTCC 3121 CCCCCGCCCC GCAGCTCGCG TCGTGCAGGA CGTGACAAAT GGAAGTAGCA CGTCTCACTA 3181 GTCTCGTGCA GATGGACAGC ACCGCTGAGC AATGGAAGCG GGTAGGCCTT TGGGGCAGCG 3241 GCCAATAGCA GCTTTGCTCC TTCGCTTTCT GGGCTCAGAG GCTGGGAAGG GGTGGGTCCG 3301 GGGGCGGGCT CAGGGGCGGG CTCAGGGGCG GGGCGGGCGC CCGAAGGTCC TCCGGAGGCC 3361 CGGCATTCTG CACGCTTCAA AAGCGCACGT CTGCCGCGCT GTTCTCCTCT TCCTCATCTC 3421 CGGGCCTTTC GACCTGCAGC CCAAGCTTAC CATGACCGAG TACAAGCCCA CGGTGCGCCT 3481 CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA 3541 CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA 3601 AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG 3661 CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA 3721 GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA 3781 AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT 3841 CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC 3901 GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT 3961 CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC 4021 CTGGTGCATG ACCCGCAAGC CCGGTGCCTG ACGCCCGCCC CACGACCCGC AGCGCCCGAC 4081 CGAAAGGAGC GCACGACCCC ATGCATCGAT AAAATAAAAG ATTTTATTTA GTCTCCAGAA 4141 AAAGGGGGGA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT 4201 TTGCAAGGCA TGGAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTTAGGAACA 4261 GAGAGACAGC AGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT 4321 CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCCGCCC TCAGCAGTTT CTAGAGAACC 4381 ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA 4441 CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA 4501 GCCCACAACC CCTCACTCGG CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG 4561 TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG 4621 GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCATGGGTA ACAGTTTCTT 4681 GAAGTTGGAG AACAACATTC TGAGGGTAGG AGTCGAATAT TAAGTAATCC TGACTCAATT 4741 AGCCACTGTT TTGAATCCAC ATACTCCAAT ACTCCTGAAA TAGTTCATTA TGGACAGCGC 4801 AGAAAGAGCT GGGGAGAATT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 4861 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 4921 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA 4981 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC 5041 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 5101 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 5161 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 5221 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 5281 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 5341 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 5401 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 5461 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 5521 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 5581 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT 5641 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 5701 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 5761 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 5821 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 5881 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 5941 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 6001 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 6061 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG 6121 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 6181 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 6241 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 6301 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 6361 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 6421 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 6481 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 6541 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 6601 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 6661 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 6721 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 6781 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 6841 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 6901 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC 6961 TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT 7021 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG 7081 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 7141 GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA 7201 GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG 7261 CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG 7321 CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA 7381 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCGCAAG GAATGGTGCA TGCAAGGAGA 7441 TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC 7501 TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC 7561 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGCGAT 7621 TAGTCCAATT TGTTAAAGAC AGGATATCAG TGGTCCAGGC TCTAGTTTTG ACTCAACAAT 7681 ATCACCAGCT GAAGCCTATA GAGTACGAGC CATAGATAAA ATAAAAGATT TTATTTAGTC 7741 TCCAGAAAAA GGGGGGAA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.