pLV3-CMV-CHEK1-3×Myc-CopGFP-Pu

pLV3-CMV-CHEK1-3×Myc-CopGFP-Puro plasmid

PVT96356

pLV3-CMV-CHEK1-3×Myc-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CHEK1(human)-3×Myc-CopGFP-Puro
Alias: NM_001114122.3;CHK1; OZEMA21;
Gene lenght: 1431bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CHEK1-3×Myc-CopGFP-Puro plasmid Description

pLV3-CMV-CHEK1-3×Myc-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CHEK1-3×Myc-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg cagtgccctt tgtggaagac tgggacttgg tgcaaaccct 2341 gggagaaggt gcctatggag aagttcaact tgctgtgaat agagtaactg aagaagcagt 2401 cgcagtgaag attgtagata tgaagcgtgc cgtagactgt ccagaaaata ttaagaaaga 2461 gatctgtatc aataaaatgc taaatcatga aaatgtagta aaattctatg gtcacaggag 2521 agaaggcaat atccaatatt tatttctgga gtactgtagt ggaggagagc tttttgacag 2581 aatagagcca gacataggca tgcctgaacc agatgctcag agattcttcc atcaactcat 2641 ggcaggggtg gtttatctgc atggtattgg aataactcac agggatatta aaccagaaaa 2701 tcttctgttg gatgaaaggg ataacctcaa aatctcagac tttggcttgg caacagtatt 2761 tcggtataat aatcgtgagc gtttgttgaa caagatgtgt ggtactttac catatgttgc 2821 tccagaactt ctgaagagaa gagaatttca tgcagaacca gttgatgttt ggtcctgtgg 2881 aatagtactt actgcaatgc tcgctggaga attgccatgg gaccaaccca gtgacagctg 2941 tcaggagtat tctgactgga aagaaaaaaa aacatacctc aacccttgga aaaaaatcga 3001 ttctgctcct ctagctctgc tgcataaaat cttagttgag aatccatcag caagaattac 3061 cattccagac atcaaaaaag atagatggta caacaaaccc ctcaagaaag gggcaaaaag 3121 gccccgagtc acttcaggtg gtgtgtcaga gtctcccagt ggattttcta agcacattca 3181 atccaatttg gacttctctc cagtaaacag tgcttctagt gaagaaaatg tgaagtactc 3241 cagttctcag ccagaacccc gcacaggtct ttccttatgg gataccagcc cctcatacat 3301 tgataaattg gtacaaggga tcagcttttc ccagcccaca tgtcctgatc atatgctttt 3361 gaatagtcag ttacttggca ccccaggatc ctcacagaac ccctggcagc ggttggtcaa 3421 aagaatgaca cgattcttta ccaaattgga tgcagacaaa tcttatcaat gcctgaaaga 3481 gacttgtgag aagttgggct atcaatggaa gaaaagttgt atgaatcagg ttactatatc 3541 aacaactgat aggagaaaca ataaactcat tttcaaagtg aatttgttag aaatggatga 3601 taaaatattg gttgacttcc ggctttctaa gggtgatgga ttggagttca agagacactt 3661 cctgaagatt aaagggaagc tgattgatat tgtgagcagc cagaagattt ggcttcctgc 3721 cacaggatcc GAACAAAAAC TAATATCAGA GGAAGATTTG GAACAGAAAC TAATCAGTGA 3781 AGAAGATTTA GAGCAAAAGT TAATTTCCGA GGAGGACTTA TAgcggccgc Gaaggatctg 3841 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt 3901 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga 3961 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag 4021 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa 4081 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc 4141 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc 4201 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag 4261 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt 4321 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct 4381 agacgccacc atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat 4441 caccggcacc ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa 4501 gcagggccgc atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta 4561 cctgctgagc cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta 4621 cgagaacccc ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa 4681 gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt 4741 gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga 4801 caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct 4861 ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt 4921 ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc 4981 catgttcgcc ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga 5041 gtaccagcac gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa 5101 ttctgccgtg gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag 5161 tcttctaaca tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac 5221 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 5281 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 5341 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 5401 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 5461 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 5521 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 5581 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 5641 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 5701 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 5761 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa 5821 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 5881 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 5941 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 6001 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 6061 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 6121 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 6181 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 6241 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 6301 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag 6361 tcggatctcc ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc 6421 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 6481 ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 6541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6661 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6721 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 6781 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 6841 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct 6901 ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 6961 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 7021 tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg 7081 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 7141 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 7201 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 7261 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 7321 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 7381 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 7441 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 7501 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 7561 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 7621 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 7681 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 7741 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 7801 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 7861 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 7921 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 7981 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 8041 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 8101 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 8161 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 8221 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 8281 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 8341 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 8401 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 8461 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 8521 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 8581 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 8641 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 8701 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 8761 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 8821 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 8881 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 8941 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 9001 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 9061 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 9121 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 9181 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 9241 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 9301 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 9361 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 9421 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg 9481 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc 9541 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta 9601 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg 9661 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.