pLV3-CMV-ISL1-3×FLAG-mCherry-N

pLV3-CMV-ISL1-3×FLAG-mCherry-Neo plasmid

PVT94565

pLV3-CMV-ISL1-3×FLAG-mCherry-Neo plasmid Information

Plasmid name: pLV3-CMV-ISL1(human)-3×FLAG-mCherry-Neo
Alias: NM_002202.3;Isl-1; ISLET1
Gene lenght: 1047bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-ISL1-3×FLAG-mCherry-Neo plasmid Description

pLV3-CMV-ISL1-3×FLAG-mCherry-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ISL1-3×FLAG-mCherry-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgggaga catgggagat ccaccaaaaa aaaaacgtct 2341 gatttcccta tgtgttggtt gcggcaatca gattcacgat cagtatattc tgagggtttc 2401 tccggatttg gaatggcatg cggcatgttt gaaatgtgcg gagtgtaatc agtatttgga 2461 cgagagctgt acatgctttg ttagggatgg gaaaacctac tgtaaaagag attatatcag 2521 gttgtacggg atcaaatgcg ccaagtgcag catcggcttc agcaagaacg acttcgtgat 2581 gcgtgcccgc tccaaggtgt atcacatcga gtgtttccgc tgtgtggcct gcagccgcca 2641 gctcatccct ggggacgaat ttgcgcttcg ggaggacggt ctcttctgcc gagcagacca 2701 cgatgtggtg gagagggcca gtctaggcgc tggcgacccg ctcagtcccc tgcatccagc 2761 gcggccactg caaatggcag cggagcccat ctccgccagg cagccagccc tgcggcccca 2821 cgtccacaag cagccggaga agaccacccg cgtgcggact gtgctgaacg agaagcagct 2881 gcacaccttg cggacctgct acgccgcaaa cccgcggcca gatgcgctca tgaaggagca 2941 actggtagag atgacgggcc tcagtccccg tgtgatccgg gtctggtttc aaaacaagcg 3001 gtgcaaggac aagaagcgaa gcatcatgat gaagcaactc cagcagcagc agcccaatga 3061 caaaactaat atccagggga tgacaggaac tcccatggtg gctgccagtc cagagagaca 3121 cgacggtggc ttacaggcta acccagtgga agtacaaagt taccagccac cttggaaagt 3181 actgagcgac ttcgccttgc agagtgacat agatcagcct gcttttcagc aactggtcaa 3241 tttttcagaa ggaggaccgg gctctaattc cactggcagt gaagtagcat caatgtcctc 3301 tcaacttcca gatacaccta acagcatggt agccagtcct attgaggcag gatccGACTA 3361 CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA 3421 GTAgcggccg cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc 3481 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag 3541 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg 3601 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt 3661 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg 3721 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg 3781 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct 3841 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac 3901 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa 3961 gctgtgaccg gcgcctacgc tagacgccac catggtgagc aagggcgagg aggataacat 4021 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca 4081 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 4141 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt 4201 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct 4261 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 4321 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg 4381 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 4441 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct 4501 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa 4561 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa 4621 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg 4681 catggacgag ctgtacaagg gatctcgcga gggcagagga agtcttctaa catgcggtga 4741 cgtggaggag aatcccggcc ctGCCACCat ggctgaacaa gatggattgc acgcaggttc 4801 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 4861 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 4921 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 4981 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 5041 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 5101 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 5161 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 5221 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 5281 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 5341 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 5401 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 5461 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 5521 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaGTCGACA ATCAACCTCT 5581 GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT 5641 ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT 5701 TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT 5761 CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT 5821 TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC 5881 GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA 5941 CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC 6001 CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA 6061 CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC 6121 TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCgg tacctttaag accaatgact 6181 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 6241 attcactccc aacgaaGata agatctgctt tttgcttgta ctgggtctct ctggttagac 6301 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 6361 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 6421 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 6481 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 6541 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 6601 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 6661 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 6721 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 6781 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt 6841 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 6901 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 6961 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 7021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 7081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 7141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 7201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 7261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 7321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 7381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 7441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 7501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 7561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 7621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 7681 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 7741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 7801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 7861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 7921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 7981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 8041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 8101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 8161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 8221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 8281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 8341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 8401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 8461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 8521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 8581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 8641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 8701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 8761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 8821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 8881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 8941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 9001 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 9061 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 9121 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 9181 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 9241 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 9301 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 9361 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 9421 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.