pMSCV-Tfeb-EGFP-PGK-Puro plasm

pMSCV-Tfeb-EGFP-PGK-Puro plasmid

PVT96509

pMSCV-Tfeb-EGFP-PGK-Puro plasmid Information

Plasmid name: pMSCV-Tfeb(mouse)-EGFP-PGK-Puro
Alias: NM_011549.4;bHLHe35; Tcfeb
Gene lenght: 1425bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: Non
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pMSCV-Tfeb(mouse)-F：CTTGAACCTCCTCGTTCGACC
Reverse primer:
Copy number: High
Induction:

pMSCV-Tfeb-EGFP-PGK-Puro plasmid Description

pMSCV-Tfeb-EGFP-PGK-Puro This is a plasmid suitable for protein expression in mammalian cells and can also be used for retrovirus packaging.

pMSCV-Tfeb-EGFP-PGK-Puro plasmid Sequence

1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT 61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA 121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA 181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT 241 CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC 301 GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC 361 CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA 421 AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA 481 GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA 541 CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG 601 TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT 661 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA 721 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA 781 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG 841 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA 901 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG 961 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT 1021 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA 1081 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG 1141 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC 1201 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC 1261 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG 1321 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG 1381 CCCTCACTCC TTCTCTAGGC GCCGGAATTA GATCTCTCGA GCCACCatgg cgtcacgcat 1441 cgggctgcgc atgcagctca tgcgggagca ggcccagcag gaggagcagc gagagcgcat 1501 gcagcagcag gctgtcatgc attatatgca acagcagcag cagcagcagc agcagctggg 1561 tgggcccccc accccagcca tcaacacccc tgtccacttc cagtcgcccc cgcctgtgcc 1621 cggggaggtg ctgaaggtgc agtcctacct ggagaacccc acctcctacc acctgcaaca 1681 gtcccagcat cagaaggttc gggagtatct gtctgagacc tatgggaaca agtttgctgc 1741 ccacgtgagc ccagcccaag gttccccgaa gcctgcccca gcagcatccc caggggtgcg 1801 ggctggacac gtactgtcca cctcggccgg caacagtgct cccaacagtc ccatggccat 1861 gctacatatc agctccaacc ccgagaaaga gtttgatgat gtcattgaca acattatgcg 1921 cctggacagc gtgctgggct acatcaaccc tgagatgcag atgcctaaca cgctgcccct 1981 gtctagcagc cacctgaacg tgtacagcgg tgacccccag gtcacagcct ccatggtggg 2041 tgtcaccagc agctcctgcc ctgccgacct gactcagaag cgagagctaa cagatgctga 2101 gagcagagcc ctggccaagg agcggcagaa gaaagacaat cacaacctaa ttgagagaag 2161 acgcaggttc aacatcaatg accggatcaa ggagctggga atgctgatcc ccaaggccaa 2221 cgacctggac gtgcgctgga acaaaggcac catcctcaag gcctctgtgg attacatccg 2281 gaggatgcag aaggacctgc agaagtcccg ggagctggag aaccactccc ggcgcctgga 2341 gatgactaac aagcagctct ggctccgcat ccaggagctg gagatgcagg cacgcgtgca 2401 cggcctcccc accacctcgc cgtcgggtgt gaatatggcc gagctggccc agcaggtggt 2461 gaagcaagag ttgcccagtg aggatggccc aggggaggcg ctgatgctgg ggcctgaggt 2521 ccctgagcct gagcaaatgc cggctcttcc tccccaggct ccgctgccct cggccgccca 2581 gccacagtct ccgttccatc acctggactt cagccatggc ctgagctttg ggggtggggg 2641 cgacgagggg cccacaggtt accccgatac cctggggaca gagcacggct ccccattccc 2701 caacctgtcc aagaaggatc tggacttaat gctcctagat gactccctgc tccccctggc 2761 ctctgacccc ctcttttcta ccatgtctcc tgaggcctcc aaggccagca gccgccggag 2821 cagcttcagc atggaggagg gtgatgttct gaccggtGGC GGTGGAGGAA GCggcggagg 2881 tggatctatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 2941 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 3001 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 3061 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 3121 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 3181 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 3241 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 3301 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 3361 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 3421 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 3481 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 3541 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3601 caagtagGAA TTCTACCGGG TAGGGGAGGC GCTTTTCCCA AGGCAGTCTG GAGCATGCGC 3661 TTTAGCAGCC CCGCTGGGCA CTTGGCGCTA CACAAGTGGC CTCTGGCCTC GCACACATTC 3721 CACATCCACC GGTAGGCGCC AACCGGCTCC GTTCTTTGGT GGCCCCTTCG CGCCACCTTC 3781 TACTCCTCCC CTAGTCAGGA AGTTCCCCCC CGCCCCGCAG CTCGCGTCGT GCAGGACGTG 3841 ACAAATGGAA GTAGCACGTC TCACTAGTCT CGTGCAGATG GACAGCACCG CTGAGCAATG 3901 GAAGCGGGTA GGCCTTTGGG GCAGCGGCCA ATAGCAGCTT TGCTCCTTCG CTTTCTGGGC 3961 TCAGAGGCTG GGAAGGGGTG GGTCCGGGGG CGGGCTCAGG GGCGGGCTCA GGGGCGGGGC 4021 GGGCGCCCGA AGGTCCTCCG GAGGCCCGGC ATTCTGCACG CTTCAAAAGC GCACGTCTGC 4081 CGCGCTGTTC TCCTCTTCCT CATCTCCGGG CCTTTCGACC TGCAGCCCAA GCTTACCATG 4141 ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC 4201 ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGACCGC 4261 CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC 4321 GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC 4381 GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC 4441 CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC 4501 GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC 4561 GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG 4621 ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC 4681 GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGACGC 4741 CCGCCCCACG ACCCGCAGCG CCCGACCGAA AGGAGCGCAC GACCCCATGC ATCGATAAAA 4801 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT 4861 GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAATACATAA CTGAGAATAG 4921 AGAAGTTCAG ATCAAGGTTA GGAACAGAGA GACAGCAGAA TATGGGCCAA ACAGGATATC 4981 TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC 5041 CCGCCCTCAG CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA 5101 ATGACCCTGT GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC 5161 TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC 5221 GATAGACTGC GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA 5281 CTTGTGGTCT CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG 5341 GGGTCTTTCA TGGGTAACAG TTTCTTGAAG TTGGAGAACA ACATTCTGAG GGTAGGAGTC 5401 GAATATTAAG TAATCCTGAC TCAATTAGCC ACTGTTTTGA ATCCACATAC TCCAATACTC 5461 CTGAAATAGT TCATTATGGA CAGCGCAGAA AGAGCTGGGG AGAATTGTGA AATTGTTATC 5521 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 5581 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 5641 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 5701 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 5761 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 5821 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 5881 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 5941 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 6001 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 6061 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 6121 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 6181 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 6241 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 6301 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 6361 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 6421 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 6481 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 6541 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 6601 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 6661 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 6721 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 6781 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 6841 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 6901 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 6961 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 7021 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 7081 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 7141 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 7201 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 7261 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 7321 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 7381 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 7441 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 7501 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 7561 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 7621 TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA 7681 AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC 7741 TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA 7801 GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG 7861 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT 7921 GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG 7981 AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG 8041 CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG 8101 CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC 8161 CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC 8221 ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC 8281 CACGATGCGT CCGGCGTAGA GGCGATTAGT CCAATTTGTT AAAGACAGGA TATCAGTGGT 8341 CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG CCTATAGAGT ACGAGCCATA 8401 GATAAAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.