pColdI-6×His-mNeonGreen plasmi

pColdI-6×His-mNeonGreen plasmid

PVT95909

pColdI-6×His-mNeonGreen plasmid Information

Plasmid name: pColdI-6×His-mNeonGreen
Alias:
Gene lenght:
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter:
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: f1 ori-F2
Reverse primer:
Copy number: High
Induction: IPTG Induction

pColdI-6×His-mNeonGreen plasmid Description

pColdI-6×His-mNeonGreen is a plasmid suitable for protein expression in Escherichia coli.

pColdI-6×His-mNeonGreen plasmid Sequence

1 aaggaatggt gtggccgatt aatcataaat atgaaaaata attgttgcat cacccgccaa 61 tgcgtggctt aatgcacatc aaattgtgag cggataacaa tttgatgtgc tagcgcatat 121 ccagtgtagt aaggcaagtc ccttcaagag ttatcgttga tacccctcgt agtgcacatt 181 cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc gaaaggcaca cttaattatt 241 aagaggtaat acaccatgaa tcacaaagtg catcatcatc atcatcatat cgaaggtagg 301 catatggagc tcggtaccct cgagggatcc atggtgagca agggcgagga ggataacatg 361 gcctctctcc cagcgacaca tgagttacac atctttggct ccatcaacgg tgtggacttt 421 gacatggtgg gtcagggcac cggcaatcca aatgatggtt atgaggagtt aaacctgaag 481 tccaccaagg gtgacctcca gttctccccc tggattctgg tccctcatat cgggtatggc 541 ttccatcagt acctgcccta ccctgacggg atgtcgcctt tccaggccgc catggtagat 601 ggctccggat accaagtcca tcgcacaatg cagtttgaag atggtgcctc ccttactgtt 661 aactaccgct acacctacga gggaagccac atcaaaggag aggcccaggt gaaggggact 721 ggtttccctg ctgacggtcc tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg 781 tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc 841 actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca 901 atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag 961 cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc 1021 atggacgagc tgtacaagta atctagatag gtaatctctg cttaaaagca cagaatctaa 1081 gatccctgcc atttggcggg gattttttta tttgttttca ggaaataaat aatcgatcgc 1141 gtaataaaat ctattattat ttttgtgaag aataaatttg ggtgcaatga gaatgcgcag 1201 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 1261 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 1321 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 1381 actgagagtg caccataaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt 1441 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc 1501 aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 1561 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 1621 acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg 1681 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 1741 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 1801 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtactatgg 1861 ttgctttgac gtatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 1921 aggcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 1981 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 2041 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 2101 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 2161 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 2221 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 2281 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 2341 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 2401 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 2461 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 2521 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 2581 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 2641 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 2701 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 2761 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 2821 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 2881 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 2941 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 3001 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 3061 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 3121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 3181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 3241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 3301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 3361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 3421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 3481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 3541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 3601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 3661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 3721 ggccttttgc tcacatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg 3781 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg 3841 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 3901 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc 3961 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc 4021 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc 4081 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca 4141 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca 4201 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg 4261 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga 4321 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac 4381 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct 4441 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc 4501 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta 4561 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg 4621 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat 4681 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca 4741 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc 4801 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc 4861 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt 4921 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg 4981 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc 5041 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgc

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.