pT3-U6-Josd2-shRNA3-EF1a-MCS p

pT3-U6-Josd2-shRNA3-EF1a-MCS plasmid

PVT94202

pT3-U6-Josd2-shRNA3-EF1a-MCS plasmid Information

Plasmid name: pT3-U6-Josd2(mouse)-shRNA3-EF1a-MCS
Alias: GTGCTGTCCATGCTCTCAACA;
Gene lenght:
Host: Mammalian cells
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pT3-U6-Josd2-shRNA3-EF1a-MCS plasmid Description

pT3-U6-Josd2-shRNA3-EF1a-MCS This is a plasmid suitable for interference silencing in mammalian cells.

pT3-U6-Josd2-shRNA3-EF1a-MCS plasmid Sequence

1 GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG 61 GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT 121 GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA 181 GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC 241 GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT 301 ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG 361 GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG 421 CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT 481 TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG 541 CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC 601 GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA 661 GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT 721 GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA 781 GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG 841 ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG 901 TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT 961 TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG 1021 GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA 1081 TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA 1141 GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTAGCTT GGTACTAATA 1201 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGGTAAGCTT GGTACCGAGC TCGGATCCAC 1261 TAGTCCAGTG TGGTGGAATT CTGCAGATAT CAACAAGTTT GTACAAAAAA GCAGGCTCCG 1321 CGGCCGCCCC CTTCACCATG GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT 1381 GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC 1441 CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT 1501 CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA 1561 GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT 1621 CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAA TAACTTCGTA TAATGTATGC 1681 TATACGAAGT TATATCGTAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 1741 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGAATTCCT TGAAATACAT CCACAGGTAC 1801 ACCTCCAATT GACTCAAATG ATGTCAATTA GTCTATCAGA AGCTTCTAAA GCCATGACAT 1861 CATTTTCTGG AATTTTCCAA GCTGTTTAAA GGCACAGTCA ACTTAGTGTA TGTAAACTTC 1921 TGACCCACTG GAATTGTGAT ACAGTGAATT ATAAGTGAAA TAATCTGTCT GTAAACAATT 1981 GTTGGAAAAA TGACTTGTGT CATGCACAAA GTAGATGTCC TAACTGACTT GCCAAAACTA 2041 TTGTTTGTTA ACAAGAAATT TGTGGAGTAG TTGAAAAACG AGTTTTAATG ACTCCAACTT 2101 AAGTGTATGT AAACTTCCGA CTTCAACTGT AGGGTACCGA GCTCGAATTC ACTGGCCGTC 2161 GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA 2221 CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA 2281 CAGTTGCGCA GCCTGAATGG CGAATGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG 2341 TGCGGTATTT CACACCGCAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG 2401 TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC 2461 CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT 2521 TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG 2581 GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG 2641 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA 2701 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT 2761 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA 2821 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC 2881 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA 2941 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG 3001 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA 3061 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA 3121 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG 3181 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG 3241 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA 3301 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA 3361 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT 3421 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA 3481 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG 3541 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT 3601 TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT 3661 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA 3721 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA 3781 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG 3841 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC 3901 AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG 3961 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC 4021 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG 4081 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC 4141 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA 4201 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT 4261 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG 4321 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG 4381 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA 4441 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC 4501 AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC 4561 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC 4621 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA 4681 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA 4741 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC 4801 GACTCTAGAC CTATACAGTT GAAGTCGGAA GTTTACATAC ACTTAAGTTG GAGTCATTAA 4861 AACTCGTTTT TCAACTACTC CACAAATTTC TTGTTAACAA ACAATAGTTT TGGCAAGTCA 4921 GTTAGGACAT CTACTTTGTG CATGACACAA GTCATTTTTC CAACAATTGT TTACAGACAG 4981 ATTATTTCAC TTATAATTCA CTGTATCACA ATTCCAGTGG GTCAGAAGTT TACATACACT 5041 AAGTTGACTG TGCCTTTAAA CAGCTTGGAA AATTCCAGAA AATGATGTCA TGGCTTTAGA 5101 AGCTTCTGAT AGACTAATTG ACATCATTTG AGTCAATTGG AGGTGTACCT GTGGATGTAT 5161 TTCAAGGGGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 5221 TGCAGCGGCC GCTAGCGAGG GCCTATTTCC CATGATTCCT TCATATTTGC ATATACGATA 5281 CAAGGCTGTT AGAGAGATAA TTAGAATTAA TTTGACTGTA AACACAAAGA TATTAGTACA 5341 AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA AATTATGTTT 5401 TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 5461 TATCTTGTGG AAAGGACgaa ttcgGTGCTG TCCATGCTCT CAACATTCAA GAGATGTTGA 5521 GAGCATGGAC AGCACTTTTT TGCTAGCTCG AGATCTTAAT TAAACTAGTA TCGATATAAC 5581 TTCGTATAAT GTATGCTATA CGAAGTTATA TGTACGGGCC AGATATACGC GTTGACATTG 5641 ATTATTGACT AGGCTTTTGC AAAAAGCTTT GCAAAGATGG ATAAAGTTTT AAACAGAGAG 5701 GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA GTGGGAATT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.