pLV2-CMV-HS3ST4-3×FLAG-Puro pl

pLV2-CMV-HS3ST4-3×FLAG-Puro plasmid

PVT90853

pLV2-CMV-HS3ST4-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-HS3ST4(human)-3×FLAG-Puro
Alias: NM_006040.3;3-OST-4; 30ST4; 3OST4; h3-OST-4;
Gene lenght: 1368bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-HS3ST4-3×FLAG-Puro plasmid Description

pLV2-CMV-HS3ST4-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-HS3ST4-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggcccg gtggcccgca cctcctccgc ctccgcctcc 2881 gcctccacct ctggccgcgc cgccgccgcc cggcgcctct gctaaggggc cgccggcgcg 2941 caagctgctt tttatgtgca ccttgtccct gtctgtcacc tacctgtgct acagcctcct 3001 gggcggctcg ggctccctgc aattccctct ggcgctgcag gagtcgccgg gcgccgccgc 3061 cgagcccccg ccgagcccgc cgccaccctc tctgctgcct acccccgtgc gcctcggcgc 3121 cccctcgcag ccgcccgcgc cgccgccgct ggacaacgcg agccacgggg agccgcccga 3181 gcccccagag cagccagccg cccccgggac cgacggctgg gggctgccga gcggcggcgg 3241 aggcgcccag gacgcctggc tccggacccc gctggccccc agcgagatga tcacggctca 3301 gagcgcgctg ccggagaggg aagcgcagga gtccagcacc accgacgagg atctcgcagg 3361 ccggagagcg gccaacggga gcagcgagag gggcggcgcc gtcagcaccc ccgactatgg 3421 ggagaagaag ctgccacagg cgctcatcat cggggtcaag aaaggaggga cccgcgcgct 3481 gctggaggcg atccgcgtgc acccggacgt gcgggcggtg ggcgtagagc cgcacttctt 3541 cgacaggaac tacgaaaagg ggttggagtg gtacagaaat gtgatgccca agactttgga 3601 tgggcaaata accatggaga agactccaag ttactttgtg acaaatgagg ctcccaagcg 3661 cattcactcc atggccaagg acatcaaact gattgtggtg gtgagaaacc ccgtgaccag 3721 ggccatctct gactacacgc agacactgtc aaagaaaccc gagatcccca cctttgaggt 3781 gctggccttc aaaaaccgga ccctcgggct gatcgatgct tcctggagtg ccattcgaat 3841 agggatctat gcgctgcatc tggaaaactg gctccagtat ttccccctct cccagatcct 3901 ctttgtcagt ggtgagcgac tcattgtgga ccccgccggg gaaatggcca aagtacagga 3961 ttttctaggc ctcaaacgtg ttgtgactga gaagcatttc tatttcaaca aaaccaaggg 4021 gttcccttgc ctaaagaagc cagaagacag cagtgccccg aggtgcttag gcaagagcaa 4081 aggtcggact catcctcgca ttgacccaga tgtcatccac agactgagga aattctacaa 4141 acccttcaac ttgatgtttt accaaatgac tggtcaagat tttcagtggg aacaggaaga 4201 gggtgataaa GGTGGAGGCg gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA 4261 TGACATCGAC TACAAGGATG ACGATGACAA Gtagtctaga taattctacc gggtagggga 4321 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg 4381 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc 4441 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc 4501 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag 4561 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg 4621 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg 4681 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc 4741 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc 4801 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc 4861 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 4921 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 4981 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5041 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5101 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5161 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5221 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5281 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5341 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5401 tggtgcatga cccgcaagcc cggtgcctga ccgcgtctgg aacaatcaac ctctggatta 5461 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 5521 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 5581 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 5641 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 5701 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 5761 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 5821 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 5881 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 5941 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 6001 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta 6061 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg 6121 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga 6181 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg 6241 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca 6301 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg 6361 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat 6421 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag 6481 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag 6541 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct 6601 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc 6661 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc 6721 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6781 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6841 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 6901 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg 6961 ttttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 7021 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 7081 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 7141 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 7201 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 7261 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 7321 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 7381 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 7441 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 7501 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 7561 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 7621 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 7681 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 7741 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 7801 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 7861 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 7921 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 7981 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 8041 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 8101 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 8161 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 8221 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 8281 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 8341 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 8401 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 8461 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 8521 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 8581 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 8641 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 8701 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 8761 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 8821 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 8881 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 8941 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 9001 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 9061 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 9121 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg 9181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 9241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 9301 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac 9361 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa 9421 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt 9481 aaagaaattg tatttgttaa atatgtacta caaacttagt agt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.