pLV3-CMV-3×FLAG-MCS-TurboID-EF

pLV3-CMV-3×FLAG-MCS-TurboID-EF1a-mCherry-Puro plasmid

PVT95767

pLV3-CMV-3×FLAG-MCS-TurboID-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-3×FLAG-MCS-TurboID-EF1a-mCherry-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×FLAG-MCS-TurboID-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-3×FLAG-MCS-TurboID-EF1a-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-3×FLAG-MCS-TurboID-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggacta caaagaccat gacggtgatt ataaagatca 2341 tgacatcgac tacaaggatg acgatgacaa gctcgagGGC GGCGGCGGCA GCGGCGGCGG 2401 CGGCAGCgct agcaaagaca atactgtgcc tctgaagctg atcgctctcc tggctaatgg 2461 cgagttccat agtggcgaac agctgggaga aaccctgggc atgtccaggg ccgctatcaa 2521 caagcacatt cagactctgc gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg 2581 ctactctctg cccgagccta tcccgctgct gaacgctaaa cagattctgg gacagctgga 2641 cggcgggagc gtggcagtcc tgcctgtggt cgactccacc aatcagtacc tgctggatcg 2701 aatcggcgag ctgaagagtg gggatgcttg cattgcagaa tatcagcagg cagggagagg 2761 aagcagaggg aggaaatggt tctctccttt tggagctaac ctgtacctga gtatgttttg 2821 gcgcctgaag cggggaccag cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc 2881 agaagcgctg cgaaagctgg gagcagacaa ggtgcgagtc aaatggccca atgacctgta 2941 tctgcaggat agaaagctgg caggcatcct ggtggagctg gccggaataa caggcgatgc 3001 tgcacagatc gtcattggcg ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt 3061 ggtcaatcag ggctggatca cactgcagga agcagggatt aacctggaca ggaatactct 3121 ggccgctacg ctgatccgag agctgcgggc agccctggaa ctgttcgagc aggaaggcct 3181 ggctccatat ctgccacggt gggagaagct ggataacttc atcaatagac ccgtgaagct 3241 gatcattggg gacaaagaga ttttcgggat tagccggggg attgataaac agggagccct 3301 gctgctggaa caggacggag ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc 3361 tgccgaaaag taggatccgc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg 3421 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac 3481 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg 3541 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct 3601 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc 3661 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc 3721 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag 3781 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc 3841 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg 3901 ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg tgagcaaggg 3961 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 4021 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 4081 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 4141 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 4201 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 4261 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta 4321 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 4381 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 4441 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 4501 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 4561 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 4621 ccactccacc ggcggcatgg acgagctgta caagggatct cgcgagggca gaggaagtct 4681 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt 4741 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc 4801 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga 4861 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga 4921 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt 4981 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca 5041 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt 5101 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt 5161 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct 5221 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc 5281 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat 5341 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 5401 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 5461 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 5521 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 5581 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 5641 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 5701 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 5761 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 5821 cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg 5881 gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 5941 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 6001 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 6061 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6121 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6181 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 6241 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 6301 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 6361 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 6421 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6481 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 6541 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca 6601 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 6661 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 6721 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 6781 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 6841 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 6901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 6961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 7441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 7681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 7801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 7861 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 7921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 7981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 8041 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 8101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 8161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 8221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 8281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 8341 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 8401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 8461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 8521 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 8581 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 8641 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 8701 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 8761 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 8821 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 8881 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 8941 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 9001 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat 9061 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 9121 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 9181 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.