pLV3-CMV-MCS-3×FLAG-TurboID-Co

pLV3-CMV-MCS-3×FLAG-TurboID-CopGFP-Puro plasmid

PVT98843

pLV3-CMV-MCS-3×FLAG-TurboID-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MCS-3×FLAG-TurboID-CopGFP-Puro
Alias: -
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCS-3×FLAG-TurboID-CopGFP-Puro plasmid Description

pLV3-CMV-MCS-3×FLAG-TurboID-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MCS-3×FLAG-TurboID-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gtttaGaatc ggatccGACT ACAAAGACCA 2341 TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGAAAGACAA 2401 TACTGTGCCT CTGAAGCTGA TCGCTCTCCT GGCTAATGGC GAGTTCCATA GTGGCGAACA 2461 GCTGGGAGAA ACCCTGGGCA TGTCCAGGGC CGCTATCAAC AAGCACATTC AGACTCTGCG 2521 CGACTGGGGC GTGGACGTGT TCACCGTGCC CGGAAAGGGC TACTCTCTGC CCGAGCCTAT 2581 CCCGCTGCTG AACGCTAAAC AGATTCTGGG ACAGCTGGAC GGCGGGAGCG TGGCAGTCCT 2641 GCCTGTGGTC GACTCCACCA ATCAGTACCT GCTGGATCGA ATCGGCGAGC TGAAGAGTGG 2701 GGATGCTTGC ATTGCAGAAT ATCAGCAGGC AGGGAGAGGA AGCAGAGGGA GGAAATGGTT 2761 CTCTCCTTTT GGAGCTAACC TGTACCTGAG TATGTTTTGG CGCCTGAAGC GGGGACCAGC 2821 AGCAATCGGC CTGGGCCCGG TCATCGGAAT TGTCATGGCA GAAGCGCTGC GAAAGCTGGG 2881 AGCAGACAAG GTGCGAGTCA AATGGCCCAA TGACCTGTAT CTGCAGGATA GAAAGCTGGC 2941 AGGCATCCTG GTGGAGCTGG CCGGAATAAC AGGCGATGCT GCACAGATCG TCATTGGCGC 3001 CGGGATTAAC GTGGCTATGA GGCGCGTGGA GGAAAGCGTG GTCAATCAGG GCTGGATCAC 3061 ACTGCAGGAA GCAGGGATTA ACCTGGACAG GAATACTCTG GCCGCTACGC TGATCCGAGA 3121 GCTGCGGGCA GCCCTGGAAC TGTTCGAGCA GGAAGGCCTG GCTCCATATC TGCCACGGTG 3181 GGAGAAGCTG GATAACTTCA TCAATAGACC CGTGAAGCTG ATCATTGGGG ACAAAGAGAT 3241 TTTCGGGATT AGCCGGGGGA TTGATAAACA GGGAGCCCTG CTGCTGGAAC AGGACGGAGT 3301 TATCAAACCC TGGATGGGCG GAGAAATCAG TCTGCGGTCT GCCGAAAAGT AGgcggccgc 3361 Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc 3421 ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg 3481 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac 3541 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa 3601 cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag 3661 gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa 3721 ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc 3781 tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc 3841 aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg 3901 cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc atggagatcg 3961 agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc ggcggagagg 4021 gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc gccctgacct 4081 tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc ggcacctacc 4141 ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac accaacaccc 4201 gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg 4261 ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga 4321 tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg 4381 ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc ggctactaca 4441 gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga 4501 acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac accgagctgg 4561 gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga tcccgcgctc 4621 agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga tctcgcgagg 4681 gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct atgaccgagt 4741 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg 4801 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 4861 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 4921 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 4981 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 5041 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 5101 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 5161 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 5221 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 5281 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga aatcaacctc 5341 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 5401 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 5461 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 5521 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 5581 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 5641 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 5701 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 5761 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 5821 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc 5881 ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta agaccaatga 5941 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 6001 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag 6061 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 6121 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 6181 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 6241 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact 6301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 6361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 6421 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 6481 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 6541 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc 6601 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 6661 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 6721 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 6781 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 6841 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 6901 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 6961 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 7021 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 7081 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7141 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 7201 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 7261 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 7321 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 7381 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 7441 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 7501 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 7561 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7621 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 7681 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 7741 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 7801 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 7861 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 7921 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 7981 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 8041 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 8101 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 8161 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 8221 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 8281 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 8341 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 8401 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 8461 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 8521 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 8581 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 8641 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 8701 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 8761 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 8821 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 8881 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 8941 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 9001 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 9061 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 9121 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 9181 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.